Título:	An ancient truncated duplication of the anti-Müllerian hormone receptor type 2 gene is a potential conserved master sex determinant in the Pangasiidae catfish family.
Autoria:	WEN, M. PAN, Q. JOUANNO, E. MONTFORT, J. ZAHM, M. CABAU, C. KLOPP, C. IAMPIETRO, C. ROQUES, C. BOUCHEZ, O. CASTINEL, A. DONNADIEU, C. PARRINELLO, H. PONCET, C. BELMONTE, E. GAUTIER, V. AVARRE, J. C. DUGUE, R. GUSTIANO, R. HÀ, T. T. T. CAMPET, M. SRIPHAIROJ, K. RIBOLLI, J. O'SULLIVAN, F. L. A. DESVIGNES, T. POSTLETHWAIT, J. H. BUCAO, C. F. ROBINSON-RECHAVI, M. BOBE, J. HERPIN, A. GUIGUEN, Y.
Afiliação:	MING WEN, State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University; QIAOWEI PAN, INRAE, LPGP; ELODIE JOUANNO, INRAE, LPGP; JEROME MONTFORT, INRAE, LPGP; MARGOT ZAHM, Plate-forme bio-informatique Genotoul, Mathématiques et Informatique Appliquées de Toulouse, INRAE; CÉDRIC CABAU, SIGENAE, GenPhySE, Université de Toulouse, INRAE; CHRISTOPHE KLOPP, SIGENAE; CAROLE IAMPIETRO, INRAE; CÉLINE ROQUES, INRAE; OLIVIER BOUCHEZ, INRAE; ADRIEN CASTINEL, INRAE; CÉCILE DONNADIEU, INRAE; HUGUES PARRINELLO, Montpellier GenomiX; CHARLES PONCET, GDEC Gentyane, INRAE, Université Clermont Auvergne; ELODIE BELMONTE, GDEC Gentyane, INRAE, Université Clermont Auvergne; VÉRONIQUE GAUTIER, GDEC Gentyane, INRAE, Université Clermont Auvergne; JEAN-CHRISTOPHE AVARRE, ISEM, CNRS, IRD, Univ Montpellier; REMI DUGUE, ISEM, CNRS, IRD, Univ Montpellier; RUDHY GUSTIANO, Research Institute of Freshwater Fisheries (CRIFI-RIFF); TRÂN THI THÚY HÀ, Research Institute for Aquaculture; MARC CAMPET, Neovia Asia; KEDNAPAT SRIPHAIROJ, Faculty of Natural Resources and Agro-Industry; JOSIANE RIBOLLI, Laboratório de Biologia e Cultivo de Peixes de Água Doce, Universidade Federal de Santa Catarina; FERNANDA LOUREIRO ALMEIDA OSULLIVAN, CPAA; THOMAS DESVIGNES, Institute of Neuroscience, University of Oregon; JOHN H. POSTLETHWAIT, Institute of Neuroscience, University of Oregon; CHRISTABEL FLOI BUCAO, Department of Ecology and Evolution, University of Lausanne; MARC ROBINSON-RECHAVI, Department of Ecology and Evolution, University of Lausanne; JULIEN BOBE, INRAE, LPGP; AMAURY HERPIN, INRAE, LPGP; YANN GUIGUEN, INRAE, LPGP.
Ano de publicação:	2022
Referência:	Molecular Ecology Resources, v. 22, n. 6, p. 2411-2428, Aug. 2022.
Conteúdo:	The evolution of sex determination (SD) in teleosts is amazingly dynamic, as reflected by the variety of different master sex-determining genes identified. Pangasiids are economically important catfishes in South Asian countries, but little is known about their SD system. Here, we generated novel genomic resources for 12 Pangasiids and characterized their SD system. Based on a Pangasianodon hypophthalmus chromosome-scale genome assembly, we identified an anti-Müllerian hormone receptor type II gene (amhr2) duplication, which was further characterized as being sex-linked in males and expressed only in testes. These results point to a Y chromosome male-specific duplication (amhr2by) of the autosomal amhr2a. Sequence annotation revealed that the P. hypophthalmus Amhr2by is truncated in its N-terminal domain, lacking the cysteine-rich extracellular part of the receptor that is crucial for ligand binding, suggesting a potential route for its neofunctionalization. Reference-guided assembly of 11 additional Pangasiids, along with sex-linkage studies, revealed that this truncated amhr2by duplication is a male-specific conserved gene in Pangasiids. Reconstructions of the amhr2 phylogeny suggested that amhr2by arose from an ancient duplication/insertion event at the root of the Siluroidei radiation that is dated to ~100 million years ago. Together these results bring multiple lines of evidence supporting that amhr2by is an ancient and conserved master sex-determining gene in Pangasiids, a finding that high-lights the recurrent use of the transforming growth factor ? pathway, which is often used for the recruitment of teleost master SD genes, and provides another empirical case towards firther understanding of dynamics of SD systems.
Thesagro:	Hormônio
NAL Thesaurus:	Evolution Sex determination
Palavras-chave:	Male genome assembly Pangasiid catfishes Peixe-gato
Digital Object Identifier:	10.1111/1755-0998.13620
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPAA)