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http://www.alice.cnptia.embrapa.br/alice/handle/doc/1144491Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | NICKEL, O. | |
| dc.contributor.author | FAJARDO, T. V. M. | |
| dc.contributor.author | TARGON, M. L. N. P. | |
| dc.contributor.author | MACHADO, M. A. | |
| dc.date.accessioned | 2022-07-06T14:20:14Z | - |
| dc.date.available | 2022-07-06T14:20:14Z | - |
| dc.date.created | 2022-07-06 | |
| dc.date.issued | 2004 | |
| dc.identifier.citation | Acta Horticulturae, v. 657, p. 35-40, ISHS 2004. | |
| dc.identifier.uri | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1144491 | - |
| dc.description | The coat protein (cp) gene of Apple stem grooving Capillovirus was amplified by RT-PCR, cloned, sequenced and subcloned in the expression plasmid pMal-c2. The ASGV cp gene was expressed in E. coli as a fusion protein (fp) containing a fragment of E. coli maltose binding protein (MBP). Bacterial cells were disrupted by sonication and the ASGVcp/MBP-fp was purified by amylose resin affinity chromatography. Polyclonal antibodies from rabbits immunized with the fp, gave specific reactions to ASGV from several infected apple cultivars at dilutions of up to 1:2000 in indirect ELISA. The ASGVcp/MBP-fp reacted to antisera raised against it as well as to commercial antisera against ASGV virions in immunoblotting. These results are a fundamental contribution to the development of an efficient ASGVindexing of propagative and mother stock materials in Southern Brazil. | |
| dc.language.iso | eng | |
| dc.rights | openAccess | |
| dc.subject | ASGV | |
| dc.subject | Recombinant viral antigen | |
| dc.title | Production and Use of Polyclonal Antibodies to the Coat Protein of Apple stem grooving virus expressed in Escherichia coli. | |
| dc.type | Artigo em anais e proceedings | |
| dc.subject.thesagro | Elisa | |
| dc.subject.nalthesaurus | Apples | |
| dc.subject.nalthesaurus | Antibodies | |
| dc.subject.nalthesaurus | Recombinant antigens | |
| riaa.ainfo.id | 1144491 | |
| riaa.ainfo.lastupdate | 2022-07-06 | |
| dc.contributor.institution | OSMAR NICKEL, CNPUV | |
| dc.contributor.institution | THOR VINICIUS MARTINS FAJARDO, CNPUV | eng |
| dc.contributor.institution | M. L. N. P. TARGON, Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira | eng |
| dc.contributor.institution | M. A. MACHADO, Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira. | eng |
| Appears in Collections: | Artigo em anais de congresso (CNPUV)![]() ![]() | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Nickel-ISHS-657-2003-p35-40.pdf | 91,53 kB | Adobe PDF | ![]() View/Open |








