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dc.contributor.authorNICKEL, O.
dc.contributor.authorFAJARDO, T. V. M.
dc.contributor.authorTARGON, M. L. N. P.
dc.contributor.authorMACHADO, M. A.
dc.date.accessioned2022-07-06T14:20:14Z-
dc.date.available2022-07-06T14:20:14Z-
dc.date.created2022-07-06
dc.date.issued2004
dc.identifier.citationActa Horticulturae, v. 657, p. 35-40, ISHS 2004.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1144491-
dc.descriptionThe coat protein (cp) gene of Apple stem grooving Capillovirus was amplified by RT-PCR, cloned, sequenced and subcloned in the expression plasmid pMal-c2. The ASGV cp gene was expressed in E. coli as a fusion protein (fp) containing a fragment of E. coli maltose binding protein (MBP). Bacterial cells were disrupted by sonication and the ASGVcp/MBP-fp was purified by amylose resin affinity chromatography. Polyclonal antibodies from rabbits immunized with the fp, gave specific reactions to ASGV from several infected apple cultivars at dilutions of up to 1:2000 in indirect ELISA. The ASGVcp/MBP-fp reacted to antisera raised against it as well as to commercial antisera against ASGV virions in immunoblotting. These results are a fundamental contribution to the development of an efficient ASGVindexing of propagative and mother stock materials in Southern Brazil.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectASGV
dc.subjectRecombinant viral antigen
dc.titleProduction and Use of Polyclonal Antibodies to the Coat Protein of Apple stem grooving virus expressed in Escherichia coli.
dc.typeArtigo em anais e proceedings
dc.subject.thesagroElisa
dc.subject.nalthesaurusApples
dc.subject.nalthesaurusAntibodies
dc.subject.nalthesaurusRecombinant antigens
riaa.ainfo.id1144491
riaa.ainfo.lastupdate2022-07-06
dc.contributor.institutionOSMAR NICKEL, CNPUV
dc.contributor.institutionTHOR VINICIUS MARTINS FAJARDO, CNPUVeng
dc.contributor.institutionM. L. N. P. TARGON, Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreiraeng
dc.contributor.institutionM. A. MACHADO, Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira.eng
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