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| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.contributor.author | HOFFMANN, L. V. | |
| dc.contributor.author | BRANQUINHO, A. A. | |
| dc.contributor.author | BARROSO, P. A. V. | |
| dc.contributor.author | VASLIN, M. F. S. | |
| dc.date.accessioned | 2022-07-20T17:30:04Z | - |
| dc.date.available | 2022-07-20T17:30:04Z | - |
| dc.date.created | 2022-07-20 | |
| dc.date.issued | 2022 | |
| dc.identifier.citation | Frontiers in Plant Science, v. 13, n. 814119, 5 p. 2022. | |
| dc.identifier.isbn | 1664-462X | |
| dc.identifier.uri | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1144832 | - |
| dc.description | The cotton blue disease, caused by the cotton leafroll dwarf virus (CLRDV), leads to dwarfism, leaf rolling, and production loss in susceptible cotton varieties. To develop an enzyme-linked immunosorbent assay (ELISA) test to detect the virus in cotton and weeds, peptides based on the coat protein were used to produce polyclonal (a-GQE, a-PRN, and a-INK) and monoclonal (a-GQE, a-PRN, and a-NKF) antibodies. All six were tested as capture antibodies, and polyclonal a-GQE and the monocle onal a-NKF were labeled with the enzyme alkaline phosphatase and used as detection antibodies for a double antibody sandwich (DAS) ELISA method, in which p-nitrophenyl phosphate was added and measured by absorbance at 405 nm. The DAS-ELISA sandwich was efficient in discriminating between healthy and diseased plant extracts. The ELISA methodology detected the virus in the weeds Commelina sp., which was confirmed by RT-PCR. The monoclonal antibodies may be used to develop other diagnostic procedures. | |
| dc.language.iso | eng | |
| dc.rights | openAccess | |
| dc.subject | Enzyme-linked immunosorbent assay (ELISA) | |
| dc.subject | CLRDV | |
| dc.subject | Intermediary host | |
| dc.title | Antibodies for the coat protein of cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease. | |
| dc.type | Artigo de periódico | |
| dc.subject.thesagro | Vírus | |
| dc.subject.nalthesaurus | Serology | |
| riaa.ainfo.id | 1144832 | |
| riaa.ainfo.lastupdate | 2022-07-20 | |
| dc.identifier.doi | 10.3389/fpls.2022.814119 | |
| dc.contributor.institution | LUCIA VIEIRA HOFFMANN, CNPA; AMANDA ALVES BRANQUINHO, UNIVERSIDADE FEDERAL DE GOIÁS; PAULO AUGUSTO VIANNA BARROSO, CNPM; MAITE F. S. VASLIN, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO. | |
| Aparece en las colecciones: | Artigo em periódico indexado (CNPM)  | |
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| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| 6046.pdf | 740.45 kB | Adobe PDF |  Visualizar/Abrir |
