Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1146377
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dc.contributor.authorCARVALHEIRA, L. de R.
dc.contributor.authorSANTOS, G. B. dos
dc.contributor.authorJASMIM
dc.contributor.authorGUIMARÃES, C. R.
dc.contributor.authorCAMPOS, M. M.
dc.contributor.authorMACHADO, F. S.
dc.contributor.authorPEDROSO, A. M.
dc.contributor.authorSILVA, T. E. da
dc.contributor.authorNOGUEIRA, L. A. G.
dc.contributor.authorRODRIGUES, A. L. R.
dc.contributor.authorCARVALHO, B. C. de
dc.date.accessioned2022-09-13T14:05:39Z-
dc.date.available2022-09-13T14:05:39Z-
dc.date.created2022-09-13
dc.date.issued2022
dc.identifier.citationAnimal Reproduction, v. 19, n. 3, e20210088, 2022.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1146377-
dc.descriptionThis study evaluated the effect of crude protein (CP) reduction in four diets (156, 139, 132, and 127 g Kg-1 DM) maintaining constant metabolizable protein (188 g/day) on the follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows. Twenty-two Girolando cows with average of 21.55 ±3.19 L daily milk yield, 105.30 ±22.62 days in lactation and 3.22 ±0.03 body condition score were selected. To reduce CP in diets and maintain constant metabolizable protein, urea and soybean meal were gradually replaced by lignosulfonate-treated soybean meal (SoyPass®, Cargill), resulting in an increase in rumenundegradable protein and a reduction in rumen degradable protein. A linear and quadratic reduction was observed in the plasma and follicular fluid urea nitrogen concentration following CP reduction, with the most intense reduction occurring in the 127 g Kg-1 DM group (p<0.001). As CP reduced, there was a tendency for a linear increase in the follicular growth rate (P=0.0696), on the number and proportion of viable oocytes (P<0.09), and also a linear increase for the number (P=0.0397) and proportion (P<0.09) of grade I viable oocytes. Plus, there was a linear effect for the number of cumulus oophorus cells. Cows fed with the lowest amount of CP had cumulus-oocyte complexes with higher numbers of cumulus oophorus cells (P=0.0238). Also, the reduction of diet crude protein was followed by a decrease in the probability of oocytes? DNA degradation. In conclusion, the reduction of CP in the diet of mid-lactating Girolando cows, reduces urea nitrogen concentration in both blood plasma and follicular fluid, and, as a consequence, increases the viability of oocytes and the number of cumulus oophorus cells while reducing oocytes? DNA degradation of follicular included cumulus-oocyte complex. The reduction on dietary CP may improve in vivo oocytes-embryo development impacting fertility of lactating dairy cows.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectApoptose
dc.titleDiet crude protein reduction on follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows.
dc.typeArtigo de periódico
dc.subject.thesagroBovino
dc.subject.thesagroGado Leiteiro
dc.subject.thesagroNutrição Animal
dc.subject.thesagroReprodução Animal
dc.subject.thesagroUréia
dc.subject.nalthesaurusApoptosis
dc.subject.nalthesaurusAnimal nutrition
dc.subject.nalthesaurusAnimal reproduction
dc.subject.nalthesaurusUrea
riaa.ainfo.id1146377
riaa.ainfo.lastupdate2022-09-13
dc.identifier.doihttps://doi.org/10.1590/1984-3143-AR2021-0088
dc.contributor.institutionLUCIANO DE REZENDE CARVALHEIRA, Universidade Federal Fluminense; GUSTAVO BERVIAN DOS SANTOS, Universidade Federal Fluminense; JASMIM, Universidade Federal do Rio de Janeiro; CLÓVIS RIBEIRO GUIMARÃES, Universidade Federal de Minas Gerais; MARIANA MAGALHAES CAMPOS, CNPGL; FERNANDA SAMARINI MACHADO, CNPGL; ALEXANDRE MENDONÇA PEDROSO, AMP Consultoria; TADEU EDER DA SILVA, University of Wisconsin-Madison; LUIZ ALTAMIRO GARCIA NOGUEIRA, Universidade Federal Fluminense; ANDRÉ LUÍS RIOS RODRIGUES, Universidade Federal Fluminense; BRUNO CAMPOS DE CARVALHO, CNPGL.
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