Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148351
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Campo DCValorIdioma
dc.contributor.authorSILVA, C. K. C. da
dc.contributor.authorSANTOS, M. R. A. dos
dc.date.accessioned2022-11-16T20:01:57Z-
dc.date.available2022-11-16T20:01:57Z-
dc.date.created2022-11-16
dc.date.issued2022
dc.identifier.citationInternational Journal of Development Research, v. 12, n. 10, p. 59738-59740, Oct. 2022.
dc.identifier.issn2230-9926
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1148351-
dc.descriptionPlant tissue culture allows the clonal propagation of Coffea canephora on a large scale through somatic embryogenesis. The aim of this study was to optimize the conditions for the induction of somatic embryogenesisin leaves of the Robusta variety. The physiological stage of the leaves - drain or source; the use of the surrounding area ofprimary or secondary veins; and the position of the explants - abaxial or adaxial surface in contact with the culture medium were evaluated. The explants were inoculated in a modified MS culture medium (1/2 macro, 1/4 micronutrients), supplemented with 30 g L-1 sucrose, 6 g L-1 agar, 1.0 mg L-1 2iP and 5.0 mg L-1 IBA. At 45 days of culture, the occurrence of callus induction and the dry weight of the explants were evaluated and, at 120 days, the average number of cotyledonary embryos per explant. The leaf fragments most responsive to the induction of somatic embryogenesis were taken from drain leaves, with explants containing secondary veins, placed with the adaxial face in contact with the culture medium.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectCotyledonary embryos
dc.subjectEmbriogênese somática
dc.subjectEmbrapa Rondônia
dc.subjectPorto Velho (RO)
dc.subjectAmazônia Ocidental
dc.subjectWestern Amazon
dc.titleOptimization of somatic embryogenesis in Coffea canephora.
dc.typeArtigo de periódico
dc.subject.thesagroCafé Robusta
dc.subject.thesagroCoffea Canephora
dc.subject.thesagroCultura de Tecido
dc.subject.thesagroCampo Experimental
dc.subject.nalthesaurusTissue culture
dc.subject.nalthesaurusSomatic embryogenesis
dc.subject.nalthesaurusDemonstration farms
riaa.ainfo.id1148351
riaa.ainfo.lastupdate2022-11-16
dc.identifier.doihttps://doi.org/10.37118/ijdr.25621.10.2022
dc.contributor.institutionCAMILA KAREN CANDEIRA DA SILVA, UNIVERSIDADE FEDERAL DE RONDÔNIA; MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO.
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