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dc.contributor.authorSILVA, C. K. C. da
dc.contributor.authorSANTOS, M. R. A. dos
dc.date.accessioned2022-11-16T21:01:55Z-
dc.date.available2022-11-16T21:01:55Z-
dc.date.created2022-11-16
dc.date.issued2022
dc.identifier.citationInternational Journal of Development Research, v. 12, n. 10, p. 59734-59737, Oct. 2022.
dc.identifier.issn2230-9926
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1148353-
dc.descriptionClonal propagation of Coffea canephora varieties is feasible through somatic embryogenesis. The aim of this study was to evaluate the induction of somatic embryos from leaves of C. canephora, Robusta and Conilon varieties, in relation to different concentrations of three growth regulators. The explants were inoculated in a MS culture medium with 2iP (isopentanyladenine), IBA (indolebutyric acid) and BA (6-benzylaminopurine), in factorial combinations, at concentrations of 1.0; 2.0; 3.0; 4.0 and 5.0 mg L-1. At 45 days of culture, the percentage of callus induction was evaluated and, at 120 days, the average number of cotyledonary embryos per explant. The most efficient combinations of growth regulators to induce callogenesis in the explants were: 5.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 3.0 mg L-1 of IBA + 3. 0 mg L-1 of BA - for the Robusta variety; and 5.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 5.0 mg L-1 of IBA + 5.0 mg L-1 of BA - for the Conilon variety. As for the average number of embryos per explant, the most efficient combinations of growth regulators were: 5.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 2.0 mg L-1 of IBA + 3 .0 mg L-1 of BA ? for the Robusta cultivar; and 4.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 5.0 mg L-1 of IBA + 5.0 mg L-1 of BA - for the Conilon variety.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectCefé Conilon
dc.subjectCotyledonary embryos
dc.subjectClonal propagation
dc.subjectEmbriogênese somática
dc.subjectEmbrapa Rondônia
dc.subjectPorto Velho (RO)
dc.subjectAmazônia Ocidental
dc.subjectWestern Amazon
dc.titleInduction of somatic embryos in Robusta and Conilon varieties of Coffea canephora.
dc.typeArtigo de periódico
dc.subject.thesagroCafé Robusta
dc.subject.thesagroCoffea Canephora
dc.subject.thesagroCultura de Tecido
dc.subject.thesagroCampo Experimental
dc.subject.nalthesaurusTissue culture
dc.subject.nalthesaurusSomatic embryogenesis
dc.subject.nalthesaurusDemonstration farms
riaa.ainfo.id1148353
riaa.ainfo.lastupdate2022-11-16
dc.identifier.doihttps://doi.org/10.37118/ijdr.25620.10.2022
dc.contributor.institutionCAMILA KAREN CANDEIRA DA SILVA, UNIVERSIDADE FEDERAL DE RONDÔNIA; MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO.
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