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dc.contributor.authorARRAES, F. B. M.
dc.contributor.authorVASQUEZ, D. D. N.
dc.contributor.authorTAHIR, M.
dc.contributor.authorPINHEIRO, D. H.
dc.contributor.authorFAHEEM, M.
dc.contributor.authorFREITAS-ALVES, N. S.
dc.contributor.authorMOREIRA-PINTO, C. E.
dc.contributor.authorMOREIRA, V. J. V.
dc.contributor.authorPAES-DE-MELO, B.
dc.contributor.authorLISEI-DE-SA, M. E.
dc.contributor.authorMORGANTE, C. V.
dc.contributor.authorMOTA, A. P. Z.
dc.contributor.authorLOURENCO, I. T.
dc.contributor.authorTOGAWA, R. C.
dc.contributor.authorGRYNBERG, P.
dc.contributor.authorFRAGOSO, R. da R.
dc.contributor.authorALMEIDA-ENGLER, J. de
dc.contributor.authorLARSEN, M. R.
dc.contributor.authorGROSSI-DE-SA, M. F.
dc.date.accessioned2022-11-23T15:01:20Z-
dc.date.available2022-11-23T15:01:20Z-
dc.date.created2022-11-23
dc.date.issued2022
dc.identifier.citationPlants, v. 11, 2744, 2022.
dc.identifier.issn2223-7747
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1148619-
dc.descriptionThe root-knot nematode (RKN), Meloidogyne incognita, is a devastating soybean pathogen worldwide. The use of resistant cultivars is the most effective method to prevent economic losses caused by RKNs. To elucidate the mechanisms involved in resistance to RKN, we determined the proteome and transcriptome profiles from roots of susceptible (BRS133) and highly tolerant (PI595099) Glycine max genotypes 4, 12, and 30 days after RKN infestation. After in silico analysis, we described major defense molecules and mechanisms considered constitutive responses to nematodeinfestation, such as mTOR, PI3K-Akt, relaxin, and thermogenesis. The integrated data allowed us to identify protein families and metabolic pathways exclusively regulated in tolerant soybean genotypes. Among them, we highlighted the phenylpropanoid pathway as an early, robust, and systemic defense process capable of controlling M. incognita reproduction. Associated with this metabolic pathway, 29 differentially expressed genes encoding 11 different enzymes were identified, mainly from the flavonoid and derivative pathways. Based on differential expression in transcriptomic and proteomic data, as well as in the expression profile by RT?qPCR, and previous studies, we selected and overexpressed the GmPR10 gene in transgenic tobacco to assess its protective effect against M. incognita. Transgenic plants of the T2 generation showed up to 58% reduction in the M. incognita reproduction factor. Finally, data suggest that GmPR10 overexpression can be effective against the plant parasitic nematodeM. incognita, but its mechanism of action remains unclear. These findings will help develop new engineered soybean genotypes with higher performance in response to RKN infections.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectRoot-knot nematode
dc.subjectDifferential expression
dc.titleIntegrated omic approaches reveal molecular mechanisms of tolerance during soybean and meloidogyne incognita interactions.
dc.typeArtigo de periódico
dc.subject.thesagroMeloidogyne Incognita
dc.subject.thesagroSoja
dc.subject.thesagroGlycine Max
dc.subject.nalthesaurusTranscriptome
dc.subject.nalthesaurusProteome
dc.subject.nalthesaurusPhenylpropanoids
riaa.ainfo.id1148619
riaa.ainfo.lastupdate2022-11-23
dc.identifier.doihttps:// doi.org/10.3390/plants11202744
dc.contributor.institutionFABRICIO B. M. ARRAES, FEDERAL UNIVERSITY OF RIO GRANDE DO SUL
dc.contributor.institutionDANIEL D. N. VASQUEZ, FEDERAL UNIVERSITY OF RIO GRANDE DO SULeng
dc.contributor.institutionMUHAMMED TAHIR, UNIVERSITY OF SOUTHERN DENMARKeng
dc.contributor.institutionDANIELE H. PINHEIRO, NATIONAL INSTITUTE OF SCIENCE AND TECHNOLOGYeng
dc.contributor.institutionMUHAMMED FAHEEM, NATIONAL UNIVERSITY OF MEDICAL SCIENCES, PAKISTANeng
dc.contributor.institutionNAYARA S. FREITAS-ALVES, FEDERAL UNIVERSITY OF PARANÁeng
dc.contributor.institutionCLÍDIA E. MOREIRA-PINTO, CNPAEeng
dc.contributor.institutionVALDEIR J. V. MOREIRA, UNIVERSITY OF BRASÍLIAeng
dc.contributor.institutionBRUNO PAES-DE-MELO, CNPAEeng
dc.contributor.institutionMARIA E. LISEI-DE-SA, MINAS GERAIS AGRICULTURAL RESEARCH COMPANYeng
dc.contributor.institutionCAROLINA VIANNA MORGANTE, CPATSAeng
dc.contributor.institutionANA P. Z. MOTA, INRAEeng
dc.contributor.institutionISABELA TRISTAN LOURENCO TESSUTTI, Cenargeneng
dc.contributor.institutionROBERTO COITI TOGAWA, Cenargeneng
dc.contributor.institutionPRISCILA GRYNBERG, Cenargeneng
dc.contributor.institutionRODRIGO DA ROCHA FRAGOSO, CNPAEeng
dc.contributor.institutionJANICE DE ALMEIDA-ENGLER, INRAEeng
dc.contributor.institutionMARTIN R. LARSEN, UNIVERSITY OF SOUTHERN DENMARKeng
dc.contributor.institutionMARIA FATIMA GROSSI-DE-SA, Cenargen.eng
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