Title:	Mass spectrometry imaging for fungal interaction analysis: Classic versus imprinting methods.
Authors:	TEIXEIRA, T. S. RODRIGUES NETO, J. C. SILVA, E. A. CONCEIÇÃO, A. A. SIQUEIRA, F. G. de ABDELNUR, P. V.
Affiliation:	TALLYTA SANTOS TEIXEIRA, UNIVERSIDADE FEDERAL DE GOIÁS; JORGE CANDIDO RODRIGUES NETO, UNIVERSIDADE FEDERAL DE GOIÁS; ELIAS ALVES SILVA, CNPAE; APARECIDO ALMEIDA CONCEIÇÃO, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; PATRICIA VERARDI ABDELNUR, CNPAE.
Date Issued:	2023
Citation:	Brazilian Journal of Analytical Chemistry, v. 10, n. 38, p. 71-78, 2023.
Description:	Fungi can produce many bioactive metabolites, which are enhanced when challenged in co-culture competition. For a better evaluation of these metabolites, Mass Spectrometry Imaging (MSI) can be used to provide complementary information about the metabolite spatial localization. However, some adaptations are required on available methodologies in MSI for applications in microorganisms, particularly on sample preparation, due to the characteristics of each type of cell that has to be analyzed. The imprinting method has been shown to be a robust method when applied to sample preparation, but to our knowledge it has never been tested for microbial MALDI-MSI. Herein we applied both Classic and Imprinting MALDIMSI to compare and analyze metabolites produced by Aspergillus terreus (ATCC® 20542TM) and Pleurotus pulmonarius fungi. For the classic method, the fungi were inoculated for 8 days with PDA medium in a MALDI glass slide. For the imprinting method, fungi were also inoculated for 8 days in a MALDI glass slide and then transferred to a filter paper by manual pressure using a homemade apparatus. Samples were then dehydrated and submitted to HCCA matrix application by sublimation.
Thesagro:	Biotecnologia
NAL Thesaurus:	Aspergillus terreus Pleurotus pulmonarius Metabolomics Biotechnology
Keywords:	MALDI-MSI
DOI:	10.30744/brjac.2179-3425.AR-44-2022
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPAE)