Título:	Development and validation of an allele-specific marker for resistance to bacterial halo blight in coffea arabica.
Autoria:	ARIYOSHI, C. SERA, G. H. RODRIGUES, L. M. R. CARVALHO, F. G. SHIGUEOKA, L. H. MENDONÇA, A. E. S. PEREIRA, C. T. M. DESTÉFANO, S. A. L. PEREIRA, L. F. P.
Afiliação:	CAROLINE ARIYOSHI, UNIVERSIDADE ESTADUAL DE LONDRINA; GUSTAVO HIROSHI SERA, INSTITUTO DE DESENVOLVIMENTO RURAL DO PARANÁ; LUCAS MATEUS RIVERO RODRIGUES, INSTITUTO AGRONÔMICO; FILIPE GIMENEZ CARVALHO, INSTITUTO DE DESENVOLVIMENTO RURAL DO PARANÁ; LUCIANA HARUMI SHIGUEOKA, INSTITUTO DE DESENVOLVIMENTO RURAL DO PARANÁ; ANA ESTER SOCATELLI MENDONÇA, INSTITUTO DE DESENVOLVIMENTO RURAL DO PARANÁ; CARLOS THEODORO MOTTA PEREIRA, INSTITUTO DE DESENVOLVIMENTO RURAL DO PARANÁ; SUZETE APARECIDA LANZA DESTÉFANO, INSTITUTO BIOLÓGICO; LUIZ FILIPE PROTASIO PEREIRA, CNPCa.
Ano de publicação:	2022
Referência:	Agronomy, v. 12, n. 12, 3178, 2022.
Conteúdo:	Bacterial halo blight (BHB) is a bacterial disease, caused by Pseudomonas syringae pv. garcae, which has been gaining prominence in the main coffee-producing regions. Chemical control of this disease increases production costs and is environmentally undesirable. In this scenario, the development of new cultivars resistant to BHB is the most economical and sustainable alternative. Marker-Assisted Selection (MAS) is an appropriate strategy to assist breeding programs for resistant genotype selection. In a previous Genome-Wide Association Study (GWAS) for C. arabica and P. syringae pv. garcae interaction, we identified a locus, probably linked to qualitative resistance to the pathogen. In this work, we developed and validated a pair of Allele-Specific-Polymerase Chain Reaction (AS-PCR) primers for this locus in C. arabica breeding populations. This pair of AS-PCR primers, called Psg_QL1, was tested both in a backcross (BC) (n = 38) and in an F2 population (n = 138) segregating for resistance to BHB. The linkage between the Psg_QL1 marker and qualitative resistance showed an accuracy of 93.75%. Our results demonstrated that the Psg_QL1 marker can be applied in MAS in a robust, simple, fast, and low-cost way.
NAL Thesaurus:	Plant breeding Genetic resistance Bacterial diseases of plants Coffea arabica var. arabica
Digital Object Identifier:	https://doi.org/10.3390/agronomy12123178
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (SAPC)