Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1151045
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dc.contributor.authorALMEIDA, J. R. M. de
dc.contributor.authorWIMAN, M.
dc.contributor.authorHEER, D.
dc.contributor.authorBRINK, D. P.
dc.contributor.authorSAUER, U.
dc.contributor.authorHAHN‐HÄGERDAL, B.
dc.contributor.authorLIDÉN, G.
dc.contributor.authorGORWA‐GRAUSLUND, M. F.
dc.date.accessioned2023-01-19T20:02:46Z-
dc.date.available2023-01-19T20:02:46Z-
dc.date.created2023-01-18
dc.date.issued2023
dc.identifier.citationFermentation, v. 9, n. 72, p. 2-21, 2023.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1151045-
dc.descriptionEconomically feasible bioethanol process from lignocellulose requires efficient fermentation by yeast of all sugars present in the hydrolysate. However, when exposed to lignocellulosic hydrolysate, Saccharomyces cerevisiae is challenged with a variety of inhibitors that reduce yeast viability, growth, and fermentation rate, and in addition damage cellular structures. In order to evaluate the capability of S. cerevisiae to adapt and respond to lignocellulosic hydrolysates, the physiological effect of cultivating yeast in the spruce hydrolysate was comprehensively studied by assessment of yeast performance in simultaneous saccharification and fermentation (SSF), measurement of furaldehyde reduction activity, assessment of conversion of phenolic compounds and genome‐wide transcription analysis. The yeast cultivated in spruce hydrolysate developed a rapid adaptive response to lignocellulosic hydrolysate, which significantly improved its fermentation performance in subsequent SSF experiments. The adaptation was shown to involve the induction of NADPHdependent aldehyde reductases and conversion of phenolic compounds during the fed‐batch cultivation. These properties were correlated to the expression of several genes encoding oxidoreductases, notably AAD4, ADH6, OYE2/3, and YML131w. The other most significant transcriptional changes involved genes involved in transport mechanisms, such as YHK8, FLR1, or ATR1. A large set of genes were found to be associated with transcription factors (TFs) involved in stress response (Msn2p, Msn4p, Yap1p) but also cell growth and division (Gcr4p, Ste12p, Sok2p), and these TFs were most likely controlling the response at the post‐transcriptional level.
dc.language.isoeng
dc.rightsopenAccess
dc.titlePhysiological and molecular characterization of yeast cultures pre-adapted for fermentation of lignocellulosic hydrolysate.
dc.typeArtigo de periódico
dc.subject.nalthesaurusLignocellulose
dc.subject.nalthesaurusPhenolic compounds
dc.subject.nalthesaurusTranscriptomics
dc.subject.nalthesaurusMicroarray technology
dc.subject.nalthesaurusIndustrial microbiology
riaa.ainfo.id1151045
riaa.ainfo.lastupdate2023-01-19
dc.identifier.doihttps://doi.org/10.3390/fermentation9010072
dc.contributor.institutionJOAO RICARDO MOREIRA DE ALMEIDA, CNPAE
dc.contributor.institutionMAGNUS WIMAN, LUND UNIVERSITYeng
dc.contributor.institutionDOMINIK HEER, INSTITUTE OF MOLECULAR SYSTEMS BIOLOGYeng
dc.contributor.institutionDANIEL P. BRINK, LUND UNIVERSITYeng
dc.contributor.institutionUWE SAUER, INSTITUTE OF MOLECULAR SYSTEMS BIOLOGYeng
dc.contributor.institutionBÄRBEL HAHN‐HÄGERDAL, LUND UNIVERSITYeng
dc.contributor.institutionGUNNAR LIDÉN, LUND UNIVERSITYeng
dc.contributor.institutionMARIE F. GORWA‐GRAUSLUND, LUND UNIVERSITY.eng
Appears in Collections:Artigo em periódico indexado (CNPAE)

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