Título:	Differential allele-specific expression revealed functional variants and candidate genes related to meat quality traits in B. indicus muscle.
Autoria:	BRUSCADIN, J. J. CARDOSO, T. F. DINIZ, W. J. da S. SOUZA, M. M. de AFONSO, J. VIEIRA, D. MALHEIROS, J. M. ANDRADE, B. G. N. PETRINI, J. FERRAZ, J. B. S. ZERLOTINI NETO, A. MOURÃO, G. B. COUTINHO, L. L. REGITANO, L. C. de A.
Afiliação:	JENNIFER JESSICA BRUSCADIN, UNIVERSIDADE FEDERAL DE SÃO CARLOS; TAINÃ FIGUEIREDO CARDOSO; WELLISON JARLES DA SILVA DINIZ, AUBURN UNIVERSITY; MARCELA MARIA DE SOUZA, IOWA STATE UNIVERSITY; JULIANA AFONSO; DIELSON VIEIRA, YALE UNIVERSITY SCHOOL OF MEDICINE; JESSICA MORAES MALHEIROS, UNIVERSIDADE FEDERAL DA INTEGRAÇÃO LATINO-AMERICANA; BRUNO GABRIEL NASCIMENTO ANDRADE, MUNSTER TECHNOLOGICAL UNIVERSITY; JULIANA PETRINI, UNIVERSIDADE DE SÃO PAULO; JOSÉ BENTO STERMAN FERRAZ, UNIVERSIDADE DE SÃO PAULO; ADHEMAR ZERLOTINI NETO, CNPTIA; GERSON BARRETO MOURÃO, UNIVERSIDADE DE SÃO PAULO; LUIZ LEHMANN COUTINHO, UNIVERSIDADE DE SÃO PAULO; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.
Ano de publicação:	2022
Referência:	Genes, v. 13, n. 12, 2336, 2022.
Conteúdo:	Abstract: Traditional transcriptomics approaches have been used to identify candidate genes affecting economically important livestock traits. Regulatory variants affecting these traits, however, remain under covered. Genomic regions showing allele-specific expression (ASE) are under the effect of cis-regulatory variants, being useful for improving the accuracy of genomic selection models. Taking advantage of the better of these two methods, we investigated single nucleotide polymorphisms (SNPs) in regions showing differential ASE (DASE SNPs) between contrasting groups for beef quality traits. For these analyses, we used RNA sequencing data, imputed genotypes and genomic estimated breeding values of muscle-related traits from 190 Nelore (Bos indicus) steers. We selected 40 contrasting unrelated samples for the analysis (N = 20 animals per contrasting group) and used a beta-binomial model to identify ASE SNPs in only one group (i.e., DASE SNPs). We found 1479 DASE SNPs (FDR 0.05) associated with 55 beef-quality traits. Most DASE genes were involved with tenderness and muscle homeostasis, presenting a co-expression module enriched for the protein ubiquitination process. The results overlapped with epigenetics and phenotype-associated data, suggesting that DASE SNPs are potentially linked to cis-regulatory variants affecting simultaneously the transcription and phenotype through chromatin state modulation.
Thesagro:	Fenótipo
NAL Thesaurus:	Single nucleotide polymorphism Gene expression Phenotype Beef
Palavras-chave:	Polimorfismos de nucleotídeo único Expressão gênica Expressão alelo específica Cis-regulation ASE Allele-specific expression
Digital Object Identifier:	https://doi.org/10.3390/ genes13122336
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPPSE)