Título:	Effect of different gonadotropins on in vitro maturation of sheep oocytes.
Autoria:	BASTOS, B. D. M. SILVA, N. P. da S. GONÇALVES, P. R. CANDIDO, A. E. C. M. BARBERINO, R. de S. MONTE, A. P. O. do NOGUEIRA, D. M. CORDEIRO, M. F. MATOS, M. H. T. de LOPES JUNIOR, E. S.
Afiliação:	BRUNA DIAS MANGUEIRA BASTOS, UNIVASF; MARIA NAIARA PEREIRA DA SILVA, UNIVASF; PRICILA RAMOS GONÇALVES, UNIVASF; ANA ELISA CUSTÓDIO MONTES CÂNDIDO, UNIVASF; RICÁSSIO DE SOUSA BARBERINO, UNIVASF; ALANE PAINS OLIVEIRA DO MONTE, UNIVASF; DANIEL MAIA NOGUEIRA, CPATSA; MABEL FREITAS CORDEIRO, UNIVASF; MARIA HELENA TAVARES DE MATOS, UNIVASF; EDILSON SOARES LOPES JÚNIOR, UNIVASF.
Ano de publicação:	2022
Referência:	Semina: Ciências Agrárias, v. 43, N. 6, p. 2731-2742, nov./dez. 2022.
Conteúdo:	The aim of this study was to examine the effect of replacing the use of follicle-stimulating hormone (FSH) with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) on the in vitro maturation (IVM) of sheep oocytes. After sheep ovaries were collected (n=300), the cumulus-oocyte complexes were aspirated, selected, and divided into four groups according to the IVM medium: CON group, in which the basic IVM medium was used; and eCG, hCG, and FSH groups, in which the oocytes were immersed in basic IVM medium with 10 IU/mL eCG, 10 IU/mL hCG, and 10 μg/mL FSH-p, respectively. In vitro maturation of the oocytes was performed at 38.5 °C, in a humidified atmosphere of 5% CO2 in air, for 24 h. Subsequently, the oocytes were evaluated for the degree of cumulus-cell expansion, chromatin configuration, GSH levels, and active mitochondria. There were no significant differences for the rate of cumulus cell expansion. The percentage of oocytes in MII was higher in the eCG group than in the CON and hCG groups (P<0.05) and similar to that of the FSH group. In conclusion, eCG can be used as a substitute for FSH in IVM of sheep oocytes.
Thesagro:	Ovino Maturação Artificial Hormônio Animal
NAL Thesaurus:	Follicle-stimulating hormone Metaphase Sheep
Palavras-chave:	Hormônio folículo estimulante Metafase II Hoechst 33342 GSH Oócitos ovinos
ISSN:	1676-546X
Digital Object Identifier:	https://doi.org/10.5433/1679-0359.2022v43n6p2731
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPATSA)