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dc.contributor.authorCHABI-JESUS, C.
dc.contributor.authorRAMOS-GONZÁLEZ, P. L.
dc.contributor.authorTASSI, A. D.
dc.contributor.authorPEREIRA, L. R.
dc.contributor.authorBASTIANEL, M.
dc.contributor.authorLAU, D.
dc.contributor.authorCANALE, M. C.
dc.contributor.authorHARAKAVA, R.
dc.contributor.authorNOVELLI, V. M.
dc.contributor.authorKITAJIMA, E. W. N.
dc.contributor.authorASTUA, J. de F.
dc.date.accessioned2023-03-21T14:50:58Z-
dc.date.available2023-03-21T14:50:58Z-
dc.date.created2023-03-21
dc.date.issued2023
dc.identifier.citationPlants, v.12, n.6 p.1371. 2023.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1152548-
dc.descriptionCitrus leprosis (CL) is the main viral disease affecting the Brazilian citriculture. Sweet orange (Citrus sinensis L. Osbeck) trees affected by CL were identified in small orchards in Southern Brazil. Rod-like particles of 40 × 100 nm and electron lucent viroplasm were observed in the nucleus of infected cells in symptomatic tissues. RNA extracts from three plants, which proved negative by RT-PCR for known CL-causing viruses, were analyzed by high throughput sequencing and Sanger sequencing after RT-PCR. The genomes of bi-segmented ss(?)RNA viruses, with ORFs in a typical organization of members of the genus Dichorhavirus, were recovered. These genomes shared 98?99% nt sequence identity among them but <73% with those of known dichorhavirids, a value below the threshold for new species demarcation within that genus. Phylogenetically, the three haplotypes of the new virus called citrus bright spot virus (CiBSV) are clustered with citrus leprosis virus N, which is a dichorhavirus transmitted by Brevipalpus phoenicis sensu stricto. In CiBSV-infected citrus plants, B. papayensis and B. azores were found, but the virus could only be transmitted to Arabidopsis plants by B. azores. The study provides the first evidence of the role of B. azores as a viral vector and supports the assignment of CiBSV to the tentative new species Dichorhavirus australis.
dc.language.isoeng
dc.rightsopenAccess
dc.titleCitrus bright spot virus: a new dichorhavirus, transmitted by Brevipalpus azores, Causing Citrus Leprosis Disease in Brazil.
dc.typeArtigo de periódico
dc.subject.thesagroFruta Cítrica
dc.subject.thesagroVírus
dc.subject.nalthesaurusMononegavirales
dc.subject.nalthesaurusRhabdoviridae
dc.subject.nalthesaurusVector-borne diseases
riaa.ainfo.id1152548
riaa.ainfo.lastupdate2023-03-21
dc.identifier.doi10.3390/plants12061371
dc.contributor.institutionCAMILA CHABI-JESUS, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; PEDRO LUIS RAMOS-GONZÁLEZ, INSTITUTO BIOLÓGICO; ALINE DANIELE TASSI, INSTITUTO BIOLÓGICO; LAURA ROSSETTO PEREIRA, INSTITUTO BIOLÓGICO; MARINÊS BASTIANEL, CENTRO DE CITRICULTURA SYLVIO MOREIRA; DOUGLAS LAU, CNPT; MARIA CRISTINA CANALE, EMPRESA DE PESQUISA AGROPECUÁRIA E EXTENSÃO RURAL DE SANTA CATARINA; RICARDO HARAKAVA, INSTITUTO BIOLÓGICO; VALDENICE MOREIRA NOVELLI, CENTRO DE CITRICULTURA SYLVIO MOREIRA; ELLIOT WATA NABE KITAJIMA, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; JULIANA DE FREITAS ASTUA, CNPMF.
Appears in Collections:Artigo em periódico indexado (CNPMF)

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