Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1154066
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dc.contributor.authorPEREIRA, B. M.
dc.contributor.authorARRAES, F.
dc.contributor.authorMARTINS, A. C. Q.
dc.contributor.authorALVES, N. S. F.
dc.contributor.authorMELO, B. P.
dc.contributor.authorMORGANTE, C. V.
dc.contributor.authorSARAIVA, M. A. P.
dc.contributor.authorSA, M. F. G. de
dc.contributor.authorGUIMARAES, P. M.
dc.contributor.authorBRASILEIRO, A. C. M.
dc.date.accessioned2023-05-29T14:47:59Z-
dc.date.available2023-05-29T14:47:59Z-
dc.date.created2023-05-29
dc.date.issued2023
dc.identifier.citationPlos One, v. 18, n. 5, e0285504, 2023.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1154066-
dc.descriptionAgrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suitable for high throughput in root analysis of candidate genes in soybean.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectTransgenic soybean
dc.subjectAgrobacterium rhizogenes
dc.titleA novel soybean hairy root system for gene functional validation.
dc.typeArtigo de periódico
dc.subject.thesagroGlycine Max
dc.subject.thesagroSoja
dc.subject.thesagroMelhoramento Genético Vegetal
dc.subject.thesagroMeloidogyne spp
dc.subject.nalthesaurusSoybeans
dc.description.notesNa publicação: Maria Fátima Grossi-de-Sa; Patricia Messenberg Guimaraes.
riaa.ainfo.id1154066
riaa.ainfo.lastupdate2023-05-29
dc.identifier.doihttps://doi. org/10.1371/journal.pone.0285504
dc.contributor.institutionBRUNA MEDEIROS PEREIRA, UNIVERSIDADE DE BRASÍLIA
dc.contributor.institutionFABRÍCIO ARRAES, UNIVERSIDADE DE BRASILIAeng
dc.contributor.institutionANDRESSA CUNHA QUINTANA MARTINS, UNIVERSIDADE CATOLICA DE BRASILIAeng
dc.contributor.institutionNAYARA SABRINA FREITAS ALVES, UNIVERSIDADE FEDERAL DO PARANÁeng
dc.contributor.institutionBRUNO PAES MELO, LONGPING HIGH TECHeng
dc.contributor.institutionCAROLINA VIANNA MORGANTE, CPATSAeng
dc.contributor.institutionMARIO ALFREDO PASSOS SARAIVA, INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIAeng
dc.contributor.institutionMARIA FATIMA GROSSI DE SA, CENARGENeng
dc.contributor.institutionPATRICIA MESSEMBERG GUIMARAES, CENARGENeng
dc.contributor.institutionANA CRISTINA MIRANDA BRASILEIRO, CENARGEN.eng
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