Título:	Molecular characterization of Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis by ERIC-PCR.
Autoria:	RAMOS, C. P. DORNELES, E. M. S. HAAS, D. J. VESCHI, J. L. A. LOUREIRO, D. PORTELA, R. D. AZEVEDO, V. HEINEMANN, M. B. LAGE, A. P.
Afiliação:	CAROLINA PANTUZZA RAMOS, UFMG; ELAINE MARIA SELES DORNELES, UFLA; DIONEI JOAQUIM HAAS, UFMG; JOSIR LAINE APARECIDA VESCHI, CPATSA; DAN LOUREIRO, UFBA; RICARDO DIAS PORTELA, UFBA; VASCO AZEVEDO, UFMG; MARCOS BRYAN HEINEMANN, USP - São Paulo; ANDREY PEREIRA LAGE, UFMG.
Ano de publicação:	2022
Referência:	Ciência Rural, v. 52, n. 11, e20210328, 2022.
Conteúdo:	The aims of the present study were (i) to genotype Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis strains using enterobacterial repetitive intergenic consensus (ERIC-PCR), and (ii) to analyze the epidemiological relationships among isolates according to biovar (Equi and Ovis), species, host, and geographical origin of the C. pseudotuberculosis strains. Sixty-eight C. pseudotuberculosis, nine C. silvaticum, and one C. auriscanis, C. pseudotuberculosis ATCC® 19410? strain and the attenuated C. pseudotuberculosis 1002 vaccinal strain were fingerprinted by ERIC 1+2-PCR. Field strains were isolated from various hosts (cattle, buffaloes, sheep, goats, horses, dogs, and pigs) in six countries (Mexico, Portugal, Brazil, Equatorial Guinea, Egypt, and Israel). High genetic diversity was found among the studied Corynebacterium spp. isolates, clustering in 24 genotypes with a Hunter & Gaston diversity index (HGDI) of 0.937. The minimal spanning tree of Corynebacterium spp. revealed three clonal complexes, each associated with one bacterial species. Twenty-two genotypes were observed among C. pseudotuberculosis isolates, with an HGDI of 0.934. Three major clonal complexes were formed at the minimal spanning tree, grouped around the geographic origin of C. pseudotuberculosis isolates. These results reinforce the high typeability, epidemiological concordance, and discriminatory power of ERIC-PCR as a consistent genotyping method for C. pseudotuberculosis, which could be useful as an epidemiological tool to control caseous lymphadenitis. Moreover, our results also indicate the potential of ERIC 1+2-PCR for the genotyping of other species of Corynebacterium other than C. pseudotuberculosis.
Thesagro:	Linfadenite Caseosa Doença Animal
NAL Thesaurus:	Molecular epidemiology Caseous lymphadenitis Genotyping
Palavras-chave:	ERIC 1+2-PCR Epidemiologia molecular Genotipagem
Digital Object Identifier:	http://doi.org/10.1590/0103-8478cr20210328
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPATSA)