Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1154377
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dc.contributor.authorSADIK, M. S.eng
dc.contributor.authorHUBER, J. T.eng
dc.contributor.authorKING, K.eng
dc.contributor.authorWANDERLEY, R. D. C.eng
dc.contributor.authorDEYOUNG, D.eng
dc.contributor.authorAI-DEHNEH, A.eng
dc.contributor.authorDUDAS, C.eng
dc.date.accessioned2023-06-12T12:33:25Z-
dc.date.available2023-06-12T12:33:25Z-
dc.date.created2023-06-12
dc.date.issued1990
dc.identifier.citationJournal of Dairy Science, v. 73, n. 3, p. 694-702, mar. 1990.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1154377-
dc.descriptionThree lactating Holstein cows fitted with duodenal cannulae were fed diets containing cottonseed meal, corn gluten meal, or blood meal as protein supplements in a 3 c 3 Latin square experiment. Diets averaged 15% CP and were 60% concentrate, 31% corn silage, and 9% alfalfa hay. The flow marker was Cr2O3; the bacterial protein fraction of digesta CP was estimated by 15N (as ammonium sulfate) and diaminopimelic acid. The undegraded fraction of total feed protein entering the duodenum for respective diets was .52, .57, and .69. The 15N method was less variable than diaminopimelic acid. Based on 15N, percentage of bacterial of total protein differed among treatments (61.5, 59.4, and 55.0, respectively). Ten percent more protein entered the duodenum on blood meal than other diets, but differences were not significant. Protein sources were similar in microbial passage, but degraded protein was used most efficiently for microbial synthesis on blood meal. Incorporation of 15N consumed into bacterial protein ranged from 50 to 83% with numerically highest values on blood meal, suggesting greater efficiency of ammonia, capture. Recoveries of 15N for the 72 h as milk, feces and urine ranged from 54 to 78%.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectBacterial protein
dc.subjectDiaminopimelic acid
dc.subjectRotein degradability
dc.titleComparison of nitrogen-15 and diaminopimelic acid for estimating bacterial protein synthesis of lactating cows fed diets of varying protein degradability.
dc.typeArtigo de periódico
riaa.ainfo.id1154377
riaa.ainfo.lastupdate2023-06-12
dc.identifier.doi10.3168/jds.S0022-0302(90)78722-X
dc.contributor.institutionM. S. SADIK, University of Guyanaeng
dc.contributor.institutionJ. T. HUBER, USDA Forage Lab.eng
dc.contributor.institutionK. KING, USDA Forage Lab.eng
dc.contributor.institutionRUY DA CARVALHEIRA WANDERLEY, CPPSEeng
dc.contributor.institutionD. DEYOUNG, University of Arizonaeng
dc.contributor.institutionA. AI-DEHNEH, University of Arizonaeng
dc.contributor.institutionC. DUDAS, University of Arizona.eng
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