Por favor, use este identificador para citar o enlazar este ítem: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1154958
Registro completo de metadatos
Campo DCValorLengua/Idioma
dc.contributor.authorGOMES, T. C.
dc.contributor.authorCONRADO, R.
dc.contributor.authorOLIVEIRA, R. C. de
dc.contributor.authorSELARI, P. J. R. G.
dc.contributor.authorMELO, I. S. de
dc.contributor.authorARAÚJO, W. L.
dc.contributor.authorMARIA, D. A.
dc.contributor.authorSOUZA, A. O. de
dc.date.accessioned2023-07-17T14:27:49Z-
dc.date.available2023-07-17T14:27:49Z-
dc.date.created2023-07-17
dc.date.issued2023
dc.identifier.citationToxins v. 15, n. 5, article 344, 2023.
dc.identifier.issn2072-6651
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1154958-
dc.descriptionAbstract: This study reports the isolation and identification of the endophytic fungus Exserohilum rostratum through molecular and morphological analysis using optical and transmission electron microscopy (TEM), as well as the procurement of its secondary metabolite monocerin, an isocoumarin derivative. Considering the previously observed biological activities of monocerin, this study was performed on human umbilical vein endothelial cells (HUVECs) that are widely used as an in vitro model for several different purposes. Important parameters, such as cell viability, senescence-associated ?-galactosidase, cellular proliferation by using 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFSE), apoptosis analysis with annexin, cellular morphology through scanning electron microscopy (SEM), and laser confocal analysis were evaluated after exposing the cells to monocerin. After 24 h of exposure to monocerin at 1.25 mM, there was more than 80% of cell viability and a low percentage of cells in the early and late apoptosis and necrosis. Monocerin increased cell proliferation and did not induce cell senescence. Morphological analysis showed cellular integrity. The study demonstrates aspects of the mechanism of action of monocerin on endothelial cell proliferation, suggesting the possibility of its pharmaceutical application, such as in regenerative medicine.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectMonocerin
dc.titleEffect of Monocerin, a fungal secondary metabolite, on endothelial cells.
dc.typeArtigo de periódico
dc.subject.thesagroCróton
dc.subject.thesagroCaatinga
dc.subject.thesagroFungo
dc.subject.nalthesaurusEndophytes
dc.subject.nalthesaurusFungi
dc.subject.nalthesaurusExserohilum
dc.subject.nalthesaurusMedicinal fungi
dc.subject.nalthesaurusSecondary metabolites
dc.subject.nalthesaurusCell viability
dc.subject.nalthesaurusCell senescence
riaa.ainfo.id1154958
riaa.ainfo.lastupdate2023-07-17
dc.identifier.doihttps://doi.org/10.3390/toxins15050344
dc.contributor.institutionTAINAH COLOMBO GOMES, INSTITUTO BUTANTAN; RAFAEL CONRADO, INSTITUTO BUTANTAN; RODRIGO CARDOSO DE OLIVEIRA, UNIVERSIDADE DE SÃO PAULO; PRISCILA JANE ROMANO GONÇALVES SELARI, INSTITUTO FEDERAL GOIANO; ITAMAR SOARES DE MELO, CNPMA; WELINGTON LUIZ ARAÚJO, UNIVERSIDADE DE SÃO PAULO; DURVANEI AUGUSTO MARIA, INSTITUTO BUTANTAN; ANA OLÍVIA DE SOUZA, INSTITUTO BUTANTAN.
Aparece en las colecciones:Artigo em periódico indexado (CNPMA)

Ficheros en este ítem:
Fichero Descripción TamañoFormato 
Melo-Effect-monocerin-2022.pdf5.86 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro sencillo del ítem

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace