Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1155672
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorPEREIRA, R. S.
dc.contributor.authorCOSTA, V. V.
dc.contributor.authorGOMES, G. L. M.
dc.contributor.authorCAMPANA, P. R. V.
dc.contributor.authorPÁDUA, R. M. de
dc.contributor.authorBARBOSA, M.
dc.contributor.authorOKI, Y.
dc.contributor.authorHEIDEN, G.
dc.contributor.authorFERNANDES, G. W.
dc.contributor.authorOLIVEIRA, D. M. de
dc.contributor.authorSOUZA, D. G.
dc.contributor.authorTEIXEIRA, M. M.
dc.contributor.authorBRAGA, F. C.
dc.date.accessioned2023-08-05T00:59:16Z-
dc.date.available2023-08-05T00:59:16Z-
dc.date.created2023-08-04
dc.date.issued2022
dc.identifier.citationChemistry & Biodiversity, v. 19, n. 4, e202100842, April 2022.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1155672-
dc.descriptionZika virus (ZIKV) infection is a global threat associated to neurological disorders in adults and microcephaly in children born to infected mothers. No vaccine or drug is available against ZIKV. We herein report the anti-ZIKV activity of 36 plant extracts containing polyphenols and/or triterpenes. ZIKV-infected Vero CCL-81 cells were treated with samples at non-cytotoxic concentrations, determined by MTT and LDH assays. One third of the extracts elicited concentration-dependent anti-ZIKV effect, with viral loads reduction from 0.4 to 3.8?log units. The 12 active extracts were tested on ZIKV-infected SH-SY5Y cells and significant reductions of viral loads (in log units) were induced by Maytenus ilicifolia (4.5?log), Terminalia phaeocarpa (3.7?log), Maytenus rigida (1.7?log) and Echinodorus grandiflorus (1.7?log) extracts. Median cytotoxic concentration (CC50) of these extracts in Vero cells were higher than in SH-SY5Y lineage. M. ilicifolia (IC50=16.8±10.3??g/mL, SI=3.4) and T. phaeocarpa (IC50=22.0±6.8??g/mL, SI=4.8) were the most active extracts. UPLC-ESI-MS/MS analysis of M. ilicifolia extract led to the identification of 7 triterpenes, of which lupeol and a mixture of friedelin/friedelinol showed no activity against ZIKV. The composition of T. phaeocarpa extract comprises phenolic acids, ellagitannins and flavonoids, as recently reported by us. In conclusion, the anti-ZIKV activity of 12 plant extracts is here described for the first time and polyphenols and triterpenes were identified as the probable bioactive constituents of T. phaeocarpa and M. ilicifolia, respectively.
dc.language.isoeng
dc.rightsopenAccess
dc.titleAnti-Zika Virus Activity of Plant Extracts Containing Polyphenols and Triterpenes on Vero CCL-81 and Human Neuroblastoma SH-SY5Y Cells.
dc.typeArtigo de periódico
dc.subject.thesagroBiodiversidade
dc.subject.thesagroVírus
dc.subject.thesagroExtrato Vegetal
riaa.ainfo.id1155672
riaa.ainfo.lastupdate2023-08-04
dc.identifier.doihttps://doi.org/10.1002/cbdv.202100842
dc.contributor.institutionROSÂNGELA SANTOS PEREIRA, Universidade Federal de Minas Gerais; VIVIAN VASCONCELOS COSTA, Universidade Federal de Minas Gerais; GABRIEL LUIZ MENEZES GOMES, Universidade Federal de Minas Gerais; PRISCILLA RODRIGUES VALADARES CAMPANA, Universidade Federal de Minas Gerais; RODRIGO MAIA DE PÁDUA, Universidade Federal de Minas Gerais; MILTON BARBOSA, Universidade Federal de Minas Gerais; YUMI OKI, Universidade Federal de Minas Gerais; GUSTAVO HEIDEN, CPACT; GERALDO WILSON FERNANDES, Universidade Federal de Minas Gerais; DJALMA MENEZES DE OLIVEIRA, Universidade Estadual do Sudoeste da Bahia; DANIELE G. SOUZA, Universidade Federal de Minas Gerais; MAURO MARTINS TEIXEIRA, Universidade Federal de Minas Gerais; FERNÃO CASTRO BRAGA, Universidade Federal de Minas Gerais.
Aparece nas coleções:Artigo em periódico indexado (CPACT)

Arquivos associados a este item:
Arquivo TamanhoFormato 
Artigo-Anti8208Zika-Virus-Activity-of-Plant-Extracts.pdf1,96 MBAdobe PDFVisualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace