Título:	Biocontrol potential of actinobacteria against Pantoea ananatis, the causal agent of maize white spot disease.
Autoria:	DORNELAS, J. C. M. CARMO, P. H. F. LANA, U. G. de P. LANA, M. A. G. OLIVEIRA-PAIVA, C. A. MARRIEL, I. E.
Afiliação:	JOÃO CARLOS MAIA DORNELAS, Universidade Federal de Minas Gerais; PAULO HENRIQUE FONSECA DO CARMO, Universidade Federal de Minas Gerais; UBIRACI GOMES DE PAULA LANA, CNPMS; MARY ANE GONÇALVES LANA, Laboratório Nacional Agropecuário; CHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMS; IVANILDO EVODIO MARRIEL, CNPMS.
Ano de publicação:	2023
Referência:	Brazilian Journal of Biology, v. 83, e268015, 2023.
Conteúdo:	Pantoea ananatis is the causal agent of maize white spot, a foliar disease responsible for significant maize yield reduction worldwide, especially in Brazil. In general, the maize foliar diseases control involves the adoption of resistant genotypes and pesticides application. However, the use of agrochemicals can significantly cause increase production costs, damage to human health and negative environmental impacts. In this sense, the use of biological control agents has been considered among the most promising eco-friendly technologies for sustainable agriculture. Actinobacteria, particularly of Streptomyces genus, has been widely recognized as agroindustrially important microorganism due to its potential in producing diverse range of secondary metabolites, including antibiotics and enzymes. Thus, the aim of this work is to characterize and to evaluate the potential of soil actinobacteria for P. ananatis control. We observed that 59 actinobacteria strains (85%) exhibited proteolytic or chitinolytic activity. Only the strains Streptomyces pseudovenezuelae ACSL 470, that also exhibited high proteolytic activity, S. novaecaesareae ACSL 432 and S. laculatispora ACP 35 demonstrated high or moderate antagonist activity in vitro against P. ananatis. Temporal analysis of metabolites produced by these strains growth in different liquid media indicated greater antibacterial activity at 72 h. In this condition, chromatographic and mass spectrometry analysis revealed that S. pseudovenezuelae ACSL 470 strain produced neomycin, an aminoglycoside antibiotic that displayed high bactericidal activity in vitro against P. ananatis. This is the first report of actinobacteria acting as potential microbial antagonists for P. ananatis control. Further studies are needed to determine the control efficacy of maize white spot disease by Streptomyces strains or their metabolites in greenhouse and field conditions.
Thesagro:	Controle Biológico Antagonismo Milho Doença de Planta Folha Actinomiceto Mancha Branca
Digital Object Identifier:	https://doi.org/10.1590/1519-6984.268015
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPMS)