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dc.contributor.authorMENEZES, V. G.eng
dc.contributor.authorMONTE, A. P. O.eng
dc.contributor.authorBARBERINO, R. de S.eng
dc.contributor.authorGOUVEIA, B. B.eng
dc.contributor.authorLINS, T. L. B.eng
dc.contributor.authorMACEDO, T. J. S. eeng
dc.contributor.authorOLIVEIRA JÚNIOR, J. L. deeng
dc.contributor.authorARAÚJO, C. de A.eng
dc.contributor.authorCAMPOS, F. S.eng
dc.contributor.authorOLIVEIRA FILHO, E. F. deeng
dc.contributor.authorBATISTA, A. M.eng
dc.contributor.authorQUEIROZ, M. A. A.eng
dc.contributor.authorARAUJO, G. G. L. deeng
dc.contributor.authorMATOS, M. H. T. deeng
dc.contributor.authorWISCHRA, A.eng
dc.date.accessioned2023-09-19T17:24:21Z-
dc.date.available2023-09-19T17:24:21Z-
dc.date.created2023-09-19
dc.date.issued2023
dc.identifier.citationAnimal Reproduction Science, v. 255, 107278, Jun. 2023.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1156781-
dc.descriptionThis study evaluated the effect of reduced water intake on survival, apoptosis and immunoexpression of leptin in sheep preantral follicles, activation of primordial follicles, serum levels of leptin, estradiol (E2) and progesterone (P4), and in vitro maturation (IVM) of oocytes antral follicles, as well evaluated the effects of leptin on in vitro culture of secondary follicles isolated these animals. Ewes (n = 32) were divided into four groups: water ad libitum (Control ? 100%), 80%; 60% and 40% of ad libitum intake. Blood was collected to determine, leptin, E2 and P4, before and after experiment. After the slaughter, ovarian cortex was used to histological and immunohistochemistry analysis and oocytes IVM. Moreover, isolated secondary follicles were cultured in vitro for 12 days in control medium (&#945;-MEM+) or &#945;-MEM+ with 10 or 25 ng/mL leptin. The reduction of water intake caused a linear decreasing effect on the percentages of normal preantral follicles, especially of primordial (P < 0.05), increased the apoptosis (P < 0.05) and decreased leptin expression in preantral follicles. The treatment with 60% of water intake showed greater total growth rate of isolated secondary follicles cultured with 25 ng/L leptin (P < 0.05), compared to those cultured in &#945;-MEM+ . In conclusion, reduced water intake impaired the number of normal sheep preantral follicles, especially of primordial follicles, increased apoptosis and decreased leptin expression in preantral follicles. Moreover, secondary follicles from of ewes
dc.language.isoeng
dc.rightsopenAccess
dc.subjectFoliculogênese
dc.subjectFolículos primordiais
dc.subjectOócito
dc.subjectHormônios sexuais
dc.titleEffect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles.
dc.typeArtigo de periódico
dc.subject.thesagroOvelha
dc.subject.thesagroÁgua Potável
dc.subject.nalthesaurusDrinking water
dc.subject.nalthesaurusOocytes
dc.subject.nalthesaurusSex hormones
dc.subject.nalthesaurusSheep
riaa.ainfo.id1156781
riaa.ainfo.lastupdate2023-09-19
dc.identifier.doihttps://doi.org/10.1016/j.anireprosci.2023.107278eng
dc.contributor.institutionVANÚZIA GONÇALVES MENEZES, UNIVASFeng
dc.contributor.institutionALANE PAINS OLIVEIRA DO MONTE, UNIVASFeng
dc.contributor.institutionRICÁSSIO DE SOUSA BARBERINO, UNIVASFeng
dc.contributor.institutionBRUNA BORTOLONI GOUVEIA, UNIVASFeng
dc.contributor.institutionTHAE LANE BARBOSA GAMA LINS, UNIVASFeng
dc.contributor.institutionTAÍS JOBARD SILVA E MACEDO, UNIVASFeng
dc.contributor.institutionJOÃOZITO LIANDRO DE OLIVEIRA JÚNIOR, UNIVASFeng
dc.contributor.institutionCLEYTON DE ALMEIDA ARAÚJO, Universidade Federal do Agreste Pernambucano, Garanhuns, PEeng
dc.contributor.institutionFLEMING SENA CAMPOS, Universidade Federal do Maranh ̃ao - UFMA, Chapadina, MAeng
dc.contributor.institutionEMANUEL FELIPE DE OLIVEIRA FILHO, UFRPEeng
dc.contributor.institutionANDRÉ MARIANO BATISTA, UFRPEeng
dc.contributor.institutionMARIO ADRIANO ÁVILA QUEIROZ, UNIVASFeng
dc.contributor.institutionGHERMAN GARCIA LEAL DE ARAUJO, CPATSAeng
dc.contributor.institutionMARIA HELENA TAVARES DE MATOS, UNIVASFeng
dc.contributor.institutionAUREA WISCHRA, UFRPE.eng
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