Título:	Caracterização da função do gene FLC-LIKE durante a transição da Endo- para ecodormência na macieira.
Autoria:	SARTOR, T. FALAVIGNA, V. da S. CATTANI, A. M. SILVEIRA, C. P. MALABARBA, J. PORTO, D. D. GRYNBERG, P. TOGAWA, R. C. COSTA, M. M. do C. SANTOS, H. P. dos PASQUALI, G. REVERS, L. F.
Afiliação:	TIAGO SARTOR, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL; VÍTOR DA SILVEIRA FALAVIGNA, MAX PLANCK INSTITUTE FOR PLANT BREEDING RESEARCH; AMANDA MALVESSI CATTANI, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL; CAROLINA PEREIRA SILVEIRA, EMBRAPA UVA E VINHO; JAIANA MALABARBA, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL; DIOGO DENARDI PORTO, CPATSA; PRISCILA GRYNBERG, Cenargen; ROBERTO COITI TOGAWA, Cenargen; MARCOS MOTA DO CARMO COSTA, PARQUE ESTAÇÃO BIOLÓGICA; HENRIQUE PESSOA DOS SANTOS, CNPUV; GIANCARLO PASQUALI, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL; LUIS FERNANDO REVERS, CNPUV.
Ano de publicação:	2023
Referência:	In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 8, 2023, Florianópolis, SC. Anais...Florianópolis: SBG, 2023.
Páginas:	p. 67.
Conteúdo:	The MADS-box MdFLC-like gene was previously described within the major QTL for time of bud break in apple chromosome Chr09, and its expression gradually increases towards dormancy release. The molecular mechanisms in which MdFLC-like modulates the transition from endo- to ecodormancy and from ecodormancy to bud break are unknown. Here, the MdFLC-like gene was characterised to better understand its role during dormancy progression. The increase in MdFLC-like expression coincides with a decrease in MdDAM1 transcript levels during ecodormancy establishment. In agreement, a transactivation assay using Arabidopsis protoplasts revealed that MdFLC-like represses the GUS reporter gene controlled by the MdDAM1 promoter. Moreover, apple calli overexpressing MdFLC-like-3xFLAG showed decreased MdDAM1 expression and delayed growth, suggesting that MdFLC-like may also repress growth-related genes. A deeper characterization of the mRNA levels of MdFLC-like during bud break demonstrated that ambient temperature performs as an environmental trigger modulating its expression, which rapidly switched when temperatures changed between cold and warm. Notably, MdFLC-like and MdFT2 expression dynamically oscillated in a contrasting pattern of expression under these conditions. Our results suggest that MdFLC-like is a repressor of MdDAM1 and MdFT2 during the transition from endo- to ecodormancy and from ecodormancy to bud break, respectively, in apple trees
Thesagro:	Malus Domestica Maçã
NAL Thesaurus:	Dormancy
Palavras-chave:	FLOWERING LOCUS C DAM1 FT2
Tipo do material:	Resumo em anais e proceedings
Acesso:	openAccess
Aparece nas coleções:	Resumo em anais de congresso (CPATSA)