Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1158490
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dc.contributor.authorCOSTA, L. S. de L.eng
dc.contributor.authorALVES, N. S. de F.eng
dc.contributor.authorPINTO, C. E. M.eng
dc.contributor.authorFLORENTINO, L. H.eng
dc.contributor.authorMELO, B. P. deeng
dc.contributor.authorMOREIRA, V. J. V.eng
dc.contributor.authorSÁ, M. E. L. deeng
dc.contributor.authorARRAES, F. B. M.eng
dc.contributor.authorRECH FILHO, E. L.eng
dc.contributor.authorMORGANTE, C. V.eng
dc.contributor.authorSA, M. F. G. deeng
dc.date.accessioned2023-11-17T13:32:34Z-
dc.date.available2023-11-17T13:32:34Z-
dc.date.created2023-11-17
dc.date.issued2023
dc.identifier.citationIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 8, 2023, Florianópolis, SC. Anais... Florianópolis: SBG, 2023.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1158490-
dc.descriptionIn this study, we identified two uORFs in the 5'-UTR sequence of this gene. The entire 5' leader sequence was amplified, cloned, and site-directed mutated to delete its uORFs start codons (-ATG). The validation of these predicted uORFs was carried out in transformed Nicotiana benthamiana protoplasts using a dual-luciferase reporter vector, in which it is possible to analyze the effect of mutated and non-mutated uORFs by dividing the expression of luciferase by that of Renilla-luciferase (LUC/REN).
dc.language.isoeng
dc.rightsopenAccess
dc.subjectUORF
dc.subjectEdição do genoma
dc.subjectNematóide das galhas
dc.titleDepleção do quadro de leitura upstream como nova estratégia para manipular a tradução de gmpr10 usando crispr/cas9 para aumentar a tolerância da soja a fitonematoides.
dc.typeResumo em anais e proceedings
dc.subject.thesagroGlycine Max
dc.subject.thesagroMeloidogyne Incognita
dc.subject.thesagroSoja
dc.subject.thesagroGenomaeng
dc.subject.nalthesaurusRoot-knot nematodes
dc.format.extent2p. 131.
riaa.ainfo.id1158490
riaa.ainfo.lastupdate2023-11-17
dc.contributor.institutionLORENA SOUSA DE LOIOLA COSTA, UNIVERSIDADE FEDERAL DE BRASÍLIAeng
dc.contributor.institutionNAYARA SABRINA DE FREITAS ALVES, UNIVERSIDADE FEDERAL DO PARANÁeng
dc.contributor.institutionCLÍDIA EDUARDA MOREIRA PINTO, INSTITUTOS NACIONAIS DE CIÊNCIA E TECNOLOGIAeng
dc.contributor.institutionLILIAN HASEGAWA FLORENTINO, CENARGENeng
dc.contributor.institutionBRUNO PAES DE MELO, LONGPING HIGH TECHeng
dc.contributor.institutionVALDEIR JUNIO VAZ MOREIRAeng
dc.contributor.institutionMARIA EUGÊNIA LISEI DE SÁeng
dc.contributor.institutionFABRÍCIO BARBOSA MONTEIRO ARRAESeng
dc.contributor.institutionELIBIO LEOPOLDO RECH FILHO, CENARGENeng
dc.contributor.institutionCAROLINA VIANNA MORGANTE, CPATSAeng
dc.contributor.institutionMARIA FATIMA GROSSI DE SA, CENARGEN.eng
Appears in Collections:Resumo em anais de congresso (CPATSA)

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