Título:	Glanders Diagnosis in an Asymptomatic Mare from Brazil: Insights from Serology, Microbiological Culture, Mass Spectrometry, and Genome Sequencing.
Autoria:	SUNIGA, P. A. P. MANTOVANI, C. SANTOS, M. G. dos EGITO, A. A. do VERBISCK, N. V. SANTOS, L. R. dos DÁVILA, A. M. R. ZIMPEL, C. K. ZERPA, M. C. S. CHIEBAO, D. P. GUIMARÃES, A. M. DE S. NASSAR, A. F. DE C. ARAUJO, F. R.
Afiliação:	PAULA ADAS PEREIRA SUNIGA, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; CYNTHIA MANTOVANI, CNPGC; MARIA GORETTI DOS SANTOS, CNPGC; ANDREA ALVES DO EGITO, CNPGC; NEWTON VALERIO VERBISCK, CNPGC; LENITA RAMIRES DOS SANTOS, CNPGC; ALBERTO MARTÍN RIVERA DÁVILA, INSTITUTO OSWALDO CRUZ; CRISTINA KRAEMER ZIMPEL, MICHIGAN STATE UNIVERSITY; MARIA CAROLINA SISCO ZERPA, UNIVERSIDASDE DE SÃO PAULO; DANIELA PONTES CHIEBAO, ANIMAL HEALTH RESEARCH CENTER; ANA MÁRCIA DE SÁ GUIMARÃES, UNIVERSIDADE DE SÃO PAULO; ALESSANDRA FIGUEIREDO DE CASTRO NASSAR, ANIMAL HEALTH RESEARCH CENTER; FLABIO RIBEIRO DE ARAUJO, CNPGC.
Ano de publicação:	2023
Referência:	Pathogens, v. 12, e1250, 2023.
Conteúdo:	ABSTRACT - This manuscript elucidates the occurrence of glanders in an asymptomatic mare from Brazil presenting positive Burkholderia mallei antibody titers. The diagnosis was established through a multi-pronged approach encompassing microbiological culture, mass spectrometry, and genome sequencing. The outbreak occurred in 2019 in Tatuí, São Paulo, Brazil, and the infected mare, despite displaying no clinical symptoms, had multiple miliary lesions in the liver, as well as intense catarrhal discharge in the trachea. Samples were collected from various organs and subjected to bacterial isolation, molecular detection, and identification. The strain was identified as B. mallei using PCR and confirmed by MALDI-TOF mass spectrometry. Whole-genome sequencing revealed a genome size of 5.51 Mb with a GC content of 65.8%, 5871 genes (including 4 rRNA and 53 tRNA genes), and 5583 coding DNA sequences (CDSs). Additionally, 227 predicted pseudogenes were detected. In silico analysis of different genomic loci that allow for differentiation with Burkholderia pseudomallei confirmed the identity of the isolate as B. mallei, in addition to the characteristic genome size. The BAC 86/19 strain was identified as lineage 3, sublineage 2, which includes other strains from Brazil, India, and Iran. The genome sequencing of this strain provides valuable information that can be used to better understand the pathogen and its epidemiology, as well as to develop diagnostic tools for glanders.
Thesagro:	Doença Animal Patogenicidade
NAL Thesaurus:	Animal diseases Burkholderia mallei Genome Glanders Mass spectrometry Pathogenicity
Digital Object Identifier:	https://doi.org/10.3390/pathogens12101250
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPGC)