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dc.contributor.authorSPERANDIO, J.eng
dc.contributor.authorBASTOS, A. P. A.eng
dc.contributor.authorFONSECA, F. M. daeng
dc.contributor.authorBELLAVER, F. A. V.eng
dc.contributor.authorDAL BELLO, T. C.eng
dc.contributor.authorKUHNEN, S.eng
dc.contributor.authorMILLEZI, A. F.eng
dc.date.accessioned2023-12-06T18:12:45Z-
dc.date.available2023-12-06T18:12:45Z-
dc.date.created2023-12-06
dc.date.issued2023
dc.identifier.citationBrazilian Archives of Biology and Technology, v. 66, e23210502, 2023.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1159232-
dc.descriptionAbstract: Salmonella enterica serovar Heidelberg (SH), often isolated from broiler chicken samples, damages the entire production chain due to its high resistance in the environment. The search for sustainable disinfectant agents has intensified, focusing on the action of essential oils. This work's objective was to evaluate the in vitro, effect of Thymus vulgaris essential oil (TEO) and thymol in SH isolated from broilers, and the cytotoxicity in animal cells. Four different concentrations of TEO (0.1%, 0.2%, 0.4%, and 0.8%, (v/v)) were used against five SH isolates obtained from broilers and ATCC 8326. Thymol was evaluated at the concentrations of 0.023%, 0.047%, 0.071%, and 0.094% (v/v). In vitro antibacterial assays were performed by quantifying viable planktonic cells in the broth microdilution test. The MTT technique was used to assess the cytotoxicity in IEC-6 intestinal cells and NCTC fibroblasts. IC50 was assessed testing the concentrations of 0.0625%, 0.125%, 0.25%, and 0.5% of TEO and thymol in 24 hours. The bacterial activity was observed from the 0.2% concentration of TEO since no colony-forming units were observed. Thymol in the concentration of 0.094% controlled 83.33% of the bacteria. TEO presented an IC50 of 0.14% and 1.22%, while the IC50 for thymol was 0.068% and 0.001% for the IEC-6 and NCTC cells, respectively. On the other hand, TEO showed low cytotoxicity in fibroblasts and a potential action capacity to eliminate SH strains in vitro.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectAntibacterial activity
dc.subjectSerovar Heidelberg
dc.subjectTomilho
dc.subjectAtividade antibacteriana
dc.subjectBioprospecção Antimicrobiana
dc.titleAntimicrobial and cytotoxic bioprospection of Thymus Vulgaris and Thymol against Salmonella Enterica Serovar Heidelberg isolated in broiler chicken.
dc.typeArtigo de periódico
dc.subject.thesagroFrango de Corte
dc.subject.thesagroCadeia Produtiva
dc.subject.thesagroSalmonelose
dc.subject.thesagroSalmonella Enteritides
dc.subject.nalthesaurusBroiler chickens
dc.subject.nalthesaurusThyme
dc.subject.nalthesaurusSalmonellosis
dc.subject.nalthesaurusSalmonella enterica
dc.subject.nalthesaurusSanitizing
riaa.ainfo.id1159232
riaa.ainfo.lastupdate2023-12-06
dc.identifier.doihttps://doi.org/10.1590/1678-4324-2023210502
dc.contributor.institutionJHONATAN SPERANDIO, Instituto Federal Catarinense/Campus Concórdiaeng
dc.contributor.institutionANA PAULA ALMEIDA BASTOS, CNPSAeng
dc.contributor.institutionFRANCISCO NOE DA FONSECA, GEMeng
dc.contributor.institutionFRANCIANA A VOLPATO BELLAVER, Instituto Federal Catarinense/Campus Concórdiaeng
dc.contributor.institutionTHAIS CARLA DAL BELLO, Instituto Federal Catarinense/Campus Concórdiaeng
dc.contributor.institutionSHIRLEY KUHNEN, Universidade Federal de Santa Catarinaeng
dc.contributor.institutionALESSANDRA FARIAS MILLEZI, Instituto Federal Catarinense/Campus Concórdia.eng
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