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Title: | Production of cellulolytic and hemicellulolytic enzymes by the pectinolytic Aspergillus niger 3T5B8-C88-P83 mutant strain under different cultivation conditions. |
Authors: | CARVALHO, B. A. R.![]() ![]() ALVES, L. M. ![]() ![]() MENDES, T. D. ![]() ![]() SALUM, T. F. C. ![]() ![]() FAVARO, L. C. de L. ![]() ![]() |
Affiliation: | BRUNO ANTONIO RIBEIRO CARVALHO; LETICIA MORESCHI ALVES; THAIS DEMARCHI MENDES; THAIS FABIANA CHAN SALUM, CNPAE; LEIA CECILIA DE LIMA FAVARO, CNPAE. |
Date Issued: | 2023 |
Citation: | In: INTERNATIONAL SYMPOSIUM OF MICROBIOLOGY AND BIOTECHNOLOGY, 6., 2023, Viçosa, MG. Biotecnologia Microbiana Soluções para os grandes desafios globais: anais. Viçosa, MG: Universidade Federal de Viçosa, 2023. |
Description: | Embrapa Agroenergia has been conducting genetic improvement of filamentous fungi to increase the production of cellulases and hemicellulases to deconstruct lignocellulosic biomass. In a conventional breeding program for Aspergillus niger 3T5B8 performed in 2015, after two rounds of mutagenesis (ultraviolet followed by ethyl methanesulfonate), the pectinolytic mutant strain 3T5B8-C88-P83 was selected, which presents genetic changes in the catabolite repression by glucose pathway and a significant increase in polygalacturonase production compared to the original 3T5B8 strain. This work aimed to evaluate the production of cellulolytic and hemicellulolytic enzymes of the 3T5B8-C88-83 mutant under different cultivation conditions, seeking its future application in the enzymatic processing of cotton fabrics. Endoglucanase production by the 3T5B8-C88-P83 mutant strain was evaluated in 250 mL Erlenmeyer flasks containing different liquid and solid culture media (four repetitions). |
Thesagro: | Mutante Fermentação Solido Enzima Celulolítica Aspergillus Niger |
NAL Thesaurus: | Polygalacturonase Mutants Solid state fermentation |
Notes: | Resumo. |
Type of Material: | Resumo em anais e proceedings |
Access: | openAccess |
Appears in Collections: | Resumo em anais de congresso (CNPAE)![]() ![]() |
Files in This Item:
File | Description | Size | Format | |
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Production-of-cellulolytic-and-hemicellulolytic.pdf | 155.72 kB | Adobe PDF | ![]() View/Open |