Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160451
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorCARVALHO, M. A. de F.
dc.contributor.authorPAIVA, R.
dc.contributor.authorSTEIN, V. C.
dc.contributor.authorHERRERA, R. C.
dc.contributor.authorPORTO, J. M. P.
dc.contributor.authorVARGAS, D. P.
dc.contributor.authorALVES, E.
dc.date.accessioned2024-01-04T19:32:12Z-
dc.date.available2024-01-04T19:32:12Z-
dc.date.created2024-01-04
dc.date.issued2014
dc.identifier.citationBrazilian Archives of Biology and Technology, v. 57, n. 6, p. 851-859, 2014.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1160451-
dc.descriptionThis work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli.
dc.language.isoeng
dc.rightsopenAccess
dc.titleInduction and morpho-ultrastructural analysis of organogenic calli of a wild passionfruit.
dc.typeArtigo de periódico
dc.subject.thesagroPassifloraceae
dc.subject.nalthesaurusTissue culture
dc.subject.nalthesaurusPlant morphology
dc.subject.nalthesaurusPassion fruits
riaa.ainfo.id1160451
riaa.ainfo.lastupdate2024-01-04
dc.identifier.doihttps://doi.org/10.1590/S1516-8913201402555
dc.contributor.institutionMILENE ALVES DE FIGUEIREDO CARVALHO, CNPCa; RENATO PAIVA, UNIVERSIDADE FEDERAL DE LAVRAS; VANESSA CRISTINA STEIN, UNIVERSIDADE FEDERAL DE GOIÁS; RAÍRYS CRAVO HERRERA, UNIVERSIDADE FEDERAL DO PARÁ; JORGE MARCELO PADOVANI PORTO, INSTITUTO FEDERAL DO ESPÍRITO SANTO; DAIANE PEIXOTO VARGAS, EMBRAPA CLIMA TEMPERADO; EDUARDO ALVES, UNIVERSIDADE FEDERAL DE LAVRAS.
Aparece nas coleções:Artigo em periódico indexado (SAPC)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Induction-and-Morpho.pdf866.29 kBAdobe PDFThumbnail
Visualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace