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dc.contributor.authorSIHLER, W.
dc.contributor.authorSANCHES, M. M.
dc.contributor.authorFAZOLIN, M.
dc.contributor.authorESTRELA, J. L. V.
dc.contributor.authorSOUZA, M. L. de
dc.date.accessioned2024-02-19T17:32:17Z-
dc.date.available2024-02-19T17:32:17Z-
dc.date.created2024-02-19
dc.date.issued2024
dc.identifier.citationTechnology in Agronomy, v. 4, e001, 2024.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1162096-
dc.descriptionThe hornworm (Erinnyis ello) is an important pest of cassava and rubber tree with high capacity of migration and can be controlled with biological control agents such as baculoviruses. Baculoviruses are rod-shaped enveloped viruses with circular double-stranded DNA genomes infecting insects of the order Lepidoptera. Prior to large-scale use of certain baculovirus, it is essential to assess its genetic and morphological information. This work aimed to characterize the baculovirus isolate occurring in Erinnyis ello populations at small farms in Cruzeiro do Sul, Acre State, Brazil and to develop parameters for field management using this isolate. Viral particles were purified from infected larvae through ultracentrifugation in sucrose gradient. After treatment and negative staining with uranyl acetate 2%, viral particles were visualized using transmission electron microscope. The virus with ovicylindrical occlusion body presenting only one virion per envelope and size less than 0.5 µm was identified as a member of the genus Betabaculovirus. Viral DNA was extracted through phenol/chloroform cycles, digested with different restriction enzymes and separated with agarose gel electrophoresis. The restriction enzyme patterns obtained with the enzymes Bam HI and Hind III revealed seven and three DNA fragments, respectively; cleavage with Pst I, generated eight molar fragments and three submolar fragments and Eco RI digestion resulted in 21 fragments, with some submolar bands. Comparison of the DNA restriction pattern from ErelGV isolated in Acre with that described for ErelGV isolated from SC State showed high similarity. This isolate named as ErelGV-Acre presented potential for control of E. ello populations.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectRubber tree
dc.subjectCaterpillar
dc.subjectHornworm
dc.subjectPlagas de plantas
dc.subjectYuca
dc.subjectÁrbol de goma
dc.subjectControl biológico
dc.subjectComunidades rurales
dc.subjectCultivo a pequeña escala
dc.subjectCruzeiro do Sul (AC)
dc.subjectAcre
dc.subjectAmazônia Ocidental
dc.subjectWestern Amazon
dc.subjectAmazonia Occidental
dc.titleErinnyis ello granulovirus for cassava hornworm control in the Acre State of Brazil.
dc.typeArtigo de periódico
dc.subject.thesagroPraga de Planta
dc.subject.thesagroMandioca
dc.subject.thesagroManihot Esculenta
dc.subject.thesagroSeringueira
dc.subject.thesagroHevea Brasiliensis
dc.subject.thesagroLagarta
dc.subject.thesagroErinnyis Ello
dc.subject.thesagroControle Biológico
dc.subject.thesagroBaculovirus
dc.subject.thesagroPequeno Produtor
dc.subject.thesagroPropriedade Rural
dc.subject.nalthesaurusPlant pests
dc.subject.nalthesaurusCassava
dc.subject.nalthesaurusLepidoptera
dc.subject.nalthesaurusBiological control
dc.subject.nalthesaurusBaculoviridae
dc.subject.nalthesaurusDsDNA viruses
dc.subject.nalthesaurusRural communities
dc.subject.nalthesaurusSmall-scale farming
riaa.ainfo.id1162096
riaa.ainfo.lastupdate2024-02-19
dc.identifier.doihttps://doi.org/10.48130/tia-0023-0019
dc.contributor.institutionWILLIAM SIHLER, Cenargen; MARCIO MARTINELLO SANCHES, CNPGC; MURILO FAZOLIN, CPAF-AC; JOELMA LIMA VIDAL ESTRELA; MARLINDA LOBO DE SOUZA, Cenargen.
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