Title:	Microsatellite markers in maize: challenges and guidelines for implementing multiplex SSR analyses.
Authors:	KOLTUN, A. SILVA, P. A. da TORRES, I. Y. BONIFÁCIO-ANACLETO, F. YASSITEPE, J. E. de C. T.
Affiliation:	ALESSANDRA KOLTUN, UNIVERSIDADE ESTADUAL DE CAMPINAS; PRISCILA ALVES DA SILVA, UNIVERSIDADE ESTADUAL DE CAMPINAS; ISABELA YAMAUCHI TORRES, SEMPRE AGTECH; FERNANDO BONIFÁCIO-ANACLETO, UNIVERSIDADE DE SÃO PAULO; JULIANA ERIKA DE C T YASSITEPE, CNPTIA, UNIVERSIDADE ESTADUAL DE CAMPINAS.
Date Issued:	2024
Citation:	Crop Breeding and Applied Biotechnology, v. 24, n. 1, e46392411, 2024.
Description:	This study tested two previously constructed simple sequence repeat (SSR) sets containing ten primer pairs each (ten-plex) in 1142 maize genotypes. Here, we describe the challenges faced when implementing this microsatellite-based genotyping protocol in our laboratory and possible ways to overcome them, hopefully aiding other novice research teams in this field.
NAL Thesaurus:	Polymerase chain reaction
Keywords:	Multiplex PCR Pontuação alélica manual Interferência de fluoróforo Milho tropical Marcadores microssatélites Reação em cadeia da polimerase Manual allele scoring Pull-up Fluorophore interference Tropical maize
ISSN:	1984-7033
DOI:	http://dx.doi.org/10.1590/1984-70332024v24n1a01
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPTIA)