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dc.contributor.authorCONTRICIANI, R. E.
dc.contributor.authorGRADE, C. V. C.
dc.contributor.authorLEITE, I. B.
dc.contributor.authorVEIGA, F. C. da
dc.contributor.authorLEDUR, M. C.
dc.contributor.authorREVERTER, A.
dc.contributor.authorALEXANDRE, P. A.
dc.contributor.authorCESAR, A. S. M.
dc.contributor.authorCOUTINHO, L. L.
dc.contributor.authorALVARES, L. E.
dc.date.accessioned2024-05-29T08:21:33Z-
dc.date.available2024-05-29T08:21:33Z-
dc.date.created2024-05-28
dc.date.issued2024
dc.identifier.citationBMC Genomics, v. 25, n.168, 2024.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1164591-
dc.descriptionAbstract Background: Understanding the molecular underpinnings of phenotypic variations is critical for enhancing poultry breeding programs. The Brazilian broiler (TT) and laying hen (CC) lines exhibit striking differences in body weight, growth potential, and muscle mass. Our work aimed to compare the global transcriptome of wing and pectoral tissues during the early development (days 2.5 to 3.5) of these chicken lines, unveiling disparities in gene expression and regulation. Results: Different and bona-fide transcriptomic profiles were identified for the compared lines. A similar number of up- and downregulated differentially expressed genes (DEGs) were identified, considering the broiler line as a reference. Upregulated DEGs displayed an enrichment of protease-encoding genes, whereas downregulated DEGs exhibited a prevalence of receptors and ligands. Gene Ontology analysis revealed that upregulated DEGs were mainly associated with hormone response, mitotic cell cycle, and different metabolic and biosynthetic processes. In contrast, downregulated DEGs were primarily linked to communication, signal transduction, cell differentiation, and nervous system development. Regulatory networks were constructed for the mitotic cell cycle and cell differentiation biological processes, as their contrasting roles may impact the development of distinct postnatal traits. Within the mitotic cell cycle network, key upregulated DEGs included CCND1 and HSP90, with central regulators being NF-κB subunits (RELA and REL) and NFATC2. The cell differentiation network comprises numerous DEGs encoding transcription factors (e.g., HOX genes), receptors, ligands, and histones, while the main regulatory hubs are CREB, AR and epigenetic modifiers. Clustering analyses highlighted PIK3CD as a central player within the differentiation network. Conclusions: Our study revealed distinct developmental transcriptomes between Brazilian broiler and layer lines. The gene expression profile of broiler embryos seems to favour increased cell proliferation and delayed differentiation, which may contribute to the subsequent enlargement of pectoral tissues during foetal and postnatal development. Our findings pave the way for future functional studies and improvement of targeted traits of economic interest in poultry.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectRNA-Seq
dc.subjectGene ontology
dc.subjectRegulatory network
dc.subjectBreeding programs
dc.titlePhenotypic divergence between broiler and layer chicken lines is regulated at the molecular level during development.
dc.typeArtigo de periódico
dc.subject.thesagroGalinha Para Postura
dc.subject.thesagroPoedeira Comercial
dc.subject.thesagroFrango de Corte
dc.subject.thesagroLinhagem
dc.subject.thesagroMelhoramento Genético Animal
dc.subject.thesagroSeleção Genética
dc.subject.thesagroGene
dc.subject.nalthesaurusTranscriptome
dc.subject.nalthesaurusBroiler chickens
dc.subject.nalthesaurusLaying hens
dc.subject.nalthesaurusGene regulatory networks
riaa.ainfo.id1164591
riaa.ainfo.lastupdate2024-05-28
dc.identifier.doihttps://doi.org/10.1186/s12864-024-10083-x
dc.contributor.institutionRENATA ERBERT CONTRICIANI, UNIVERSITY OF CAMPINAS; CARLA VERMEULEN CARVALHO GRADE, UNIVERSIDADE FEDERAL DA INTEGRAÇÃO LATINO-AMERICANA; IGOR BUZZATTO-LEITE, UNIVERSITY OF CAMPINAS; FERNANDA CRISTINA DA VEIGA, UNIVERSITY OF SÃO PAULO; MONICA CORREA LEDUR, CNPSA; ANTONIO REVERTER, COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION; PAMELA ALMEIDA ALEXANDRE, COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION; ALINE SILVA MELLO CESAR, UNIVERSITY OF SÃO PAULO; LUIZ LEHMANN COUTINHO, UNIVERSITY OF SÃO PAULO; LÚCIA ELVIRA ALVARES, UNIVERSITY OF CAMPINAS.
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