Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1165405
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dc.contributor.authorMORAES, L. M. P. depor
dc.contributor.authorMARQUES, H. F.por
dc.contributor.authorREIS, V. C. B.por
dc.contributor.authorCOELHO, C. M.por
dc.contributor.authorLEITÃO, M. de C.por
dc.contributor.authorGALDINO, A. S.por
dc.contributor.authorSOUZA, T. P. P. depor
dc.contributor.authorPIVA, L. C.por
dc.contributor.authorPEREZ, A. L. A.por
dc.contributor.authorTRICHEZ, D.por
dc.contributor.authorALMEIDA, J. R. M. depor
dc.contributor.authorDE MARCO, J. L.por
dc.contributor.authorTORRES, F. A. G.por
dc.date.accessioned2024-07-03T20:55:04Z-
dc.date.available2024-07-03T20:55:04Z-
dc.date.created2024-07-03
dc.date.issued2024
dc.identifier.citationJournal of Fungi, v. 10, n. 411, 2024.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1165405-
dc.descriptionIn the context of industrial biotechnology, many bio-based products can be obtained as a result of bioprocesses using microorganisms as cell factories. Among these, yeasts stand out as one of the most important microbial platforms. Despite the fact that baker’s yeast Saccharomyces cerevisiae still occupies a relevant position as one of the main cell factories [1], the methylotrophic yeast Komagataella phaffii has gained much attention as a promising “biotech yeast” [2]. K. phaffii exhibits traits ideal for a microbial platform in biotechnological settings. This yeast shows minimal nutritional needs and grows on economical substrates, achieving cell densities exceeding 100 g·L−1 of dry cell weight [3]. Notably, it demonstrates resilience against high methanol concentrations, acidic and basic pH levels, and inhibitors derived from lignocellulosic sources, surpassing other methylotrophic yeasts [4]. Moreover, it outperforms S. cerevisiae in terms of thermo- and osmo-tolerance [2]. Its appeal as a host organism is further accentuated by efficient secretion mechanisms, yielding abundant protein secretion in bioreactor settings [5], and versatility in protein processing and posttranslational modifications [6], due to its ability to perform modifications such as O- and N-linked glycosylation and disulfide bond formation [7]. Also, K. phaffii can generate recombinant proteins either constitutively or through induction [2], facilitated by the development of numerous genetic and metabolic engineering tools alongside established fermentation processes [8]. Given its importance in modern biotechnology, in this review we present the main applications of K. phaffii in different industrial sectors. Finally, we show how modern synthetic biology approaches may be used to further optimize the utilization of K. phaffii in the context of modern biotechnology.
dc.language.isopor
dc.rightsopenAccess
dc.titleApplications of the methylotrophic yeast komagataella phaffii in the context of modern biotechnology.
dc.typeArtigo de periódico
dc.subject.nalthesaurusSynthetic biology
dc.subject.nalthesaurusBiotechnology
riaa.ainfo.id1165405
riaa.ainfo.lastupdate2024-07-03
dc.contributor.institutionLIDIA MARIA PEPE DE MORAES, UNIVERSITY OF BRASÍLIApor
dc.contributor.institutionHENRIQUE FETZNER MARQUES, UNIVERSITY OF BRASILIApor
dc.contributor.institutionVIVIANE CASTELO BRANCO REIS, UNIVERSITY OF BRASILIApor
dc.contributor.institutionCINTIA MARQUES COELHO, UNIVERSITY OF BRASILIApor
dc.contributor.institutionMATHEUS DE CASTRO LEITÃO, UNIVERSITY OF BRASILIApor
dc.contributor.institutionALEXSANDRO SOBREIRA GALDINO, FEDERAL UNIVERSITY OF SÃO JOÃO DEL-REIpor
dc.contributor.institutionTHAIS PAIVA PORTO DE SOUZA, FEDERAL UNIVERSITY OF SÃO JOÃO DEL-REIpor
dc.contributor.institutionLUIZA CESCA PIVA, UNIVERSITY OF BRASÍLIApor
dc.contributor.institutionANA LAURA ALFONSO PEREZ, UNIVERSITY OF BRASILIApor
dc.contributor.institutionDÉBORA TRICHEZ, FEDERAL UNIVERSITY OF SANTA CATARINApor
dc.contributor.institutionJOAO RICARDO MOREIRA DE ALMEIDA, CNPAEpor
dc.contributor.institutionJANICE LISBOA DE MARCO, UNIVERSITY OF BRASILIApor
dc.contributor.institutionFERNANDO ARARIPE GONÇALVES TORRES, UNIVERSITY OF BRASILIA.por
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