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dc.contributor.authorLIMA, V. H.
dc.contributor.authorMATUGAWA, A. T.
dc.contributor.authorMASCARIN, G. M.
dc.contributor.authorFERNANDES, É. K. K.
dc.date.accessioned2024-07-05T15:02:32Z-
dc.date.available2024-07-05T15:02:32Z-
dc.date.created2024-07-05
dc.date.issued2024
dc.identifier.citationMicrobiology Spectrum, v. 12, n .6, e04040-23, 2024.
dc.identifier.issn2165-0497
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1165486-
dc.descriptionAbstract: We investigated the impact of various complex organic nitrogen sources on the submerged liquid fermentation of Beauveria bassiana, a versatile entomopatho genic fungus known for producing hydrophilic yeast-like single cells called blastospores. Specifically, we examined yeast extract, autolyzed yeast, inactive yeast, cottonseed flour, corn bran, and corn gluten meal as nitrogen compounds with different carbon-to-nitro gen (C:N) ratios. Our comprehensive analysis encompassed blastospore production, tolerance to abiotic stresses, shelf stability after drying, and virulence against meal worm larvae, crucial attributes for developing effective blastospore-based biopesticides. Notably, cottonseed flour emerged as the optimal nitrogen source, yielding up to 2.5 × 109 blastospores/mL within 3 days in a bioreactor. These blastospores exhibited the highest tolerance to heat stress and UV-B radiation exposure. The endogenous C:N ratio in blastospore composition was also impacted by nitrogen sources. Bioassays with mealworm larvae demonstrated that blastospores from cottonseed flour were the most virulent, achieving faster lethality (lower LT50) and requiring a lower inoculum (LC50). Importantly, blastospores produced with cottonseed flour displayed extended viability during storage, surpassing the retention of viability compared to those from autolyzed yeast over 180 days at 4°C. Despite differences in storage viability, both nitrogen sources conferred similar long-term blastospore bioactivity against mealworms. In summary, this research advances our understanding of the crucial impact of complex organic nitrogen selection on the phenotypic traits of blastospores in association with their intracellular C:N ratio, contributing to the production of ecologically fit, shelf-stable, and virulent propagules for effective pest biocontrol programs.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectPhenotypic traits
dc.subjectThermotolerance
dc.titleComplex nitrogen sources from agro-industrial byproducts: impact on production, multi-stress tolerance, virulence, and quality of Beauveria bassiana blastospores.
dc.typeArtigo de periódico
dc.subject.thesagroBeauveria Bassiana
dc.subject.thesagroControle Biológico
dc.subject.thesagroFungo Para Controle Biológico
dc.subject.nalthesaurusBiological control agents
dc.subject.nalthesaurusEntomopathogenic fungi
dc.subject.nalthesaurusBlastospores
dc.subject.nalthesaurusUltraviolet radiation
dc.subject.nalthesaurusPlant byproducts
dc.subject.nalthesaurusYeasts
riaa.ainfo.id1165486
riaa.ainfo.lastupdate2024-07-05
dc.identifier.doihttp://dx.doi.org/10.1128/spectrum.04040-23
dc.contributor.institutionVALESCA HENRIQUE LIMA, UNIVERSIDADE FEDERAL DE GOIÁS; ALEXANDRE TOSHIHIRO MATUGAWA, UNIVERSIDADE FEDERAL DE GOIÁS; GABRIEL MOURA MASCARIN, CNPMA; ÉVERTON KORT KAMP FERNANDES, UNIVERSIDADE FEDERAL DE GOIÁS.
Aparece en las colecciones:Artigo em periódico indexado (CNPMA)

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