Title:	Growth potential and metabolite production of the filamentous fungus Trichoderma in different solid culture media.
Authors:	BARBOSA, E. T. OKURA, M. H. DIAS NETO, J. J. BARBOSA, D. B. M. VIANA, T. A. LOBO JUNIOR, M.
Affiliation:	ELDER TADEU BARBOSA, CNPAF; MONICA HITOMI OKURA, UNIVERSIDADE FEDERAL DO TRIÂNGULO MINEIRO; JUSTINO JOSE DIAS NETO; DANIELA BORGES MARQUEZ BARBOSA, UNIVERSIDADE FEDERAL DE GOIÁS; THAMYRIS ANDRADE VIANA, UNIVERSIDADE FEDERAL DE GOIÁS; MURILLO LOBO JUNIOR, CNPAF.
Date Issued:	2024
Citation:	In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA INDUSTRIAL, 1., 2024, Florianópolis. Creating connectins between biotechnology and industrial sustainability: anais. Florianópolis: Universidade Federal de Santa Catarina, 2024.
Description:	Trichoderma is a widely commercially employed filamentous fungus, notable for its utility in various industrial sectors, including pharmaceutical, agronomic, food, energy, and chemical industries. Its capacity to produce a variety of metabolites from different substrates contributes to this usefulness. This study aimed to evaluate the growth and production of secondary metabolites by a promising strain of Trichoderma in different culture media (Sabouraud Agar, Agar-Agar, Czapek Agar, Potato Dextrose Agar, Mannitol Agar, Tryptic Soy Agar, Yeast Extract Peptone Dextrose Agar, Yeast Extract Peptone Agar, and Malt Extract Agar). A disc containing Trichoderma spores and mycelium was inoculated in the center of each plate, with incubation at 25ºC for 5 days. In sequence, the treatments were compared by the diameter of the colonies. After extraction with ethyl acetate, the samples were subjected to HPLC for metabolite quantification. The Trichoderma strain showed better mycelial growth and sporulation in PDA and Malt Extract Agar. However, according to the chromatogram, the bioagent produced metabolites in the 16 - 20 minute interval, best represented by production in the Sabouraud medium. We concluded that the best substrate for metabolite production and identification is the Sabouraud culture medium.
Thesagro:	Antagonismo Trichoderma Fungo
NAL Thesaurus:	Fungal antagonists Secondary metabolites
Keywords:	HPLC
Notes:	I COBBIND.
Type of Material:	Artigo em anais e proceedings
Access:	openAccess
Appears in Collections:	Artigo em anais de congresso (CNPAF)