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dc.contributor.authorLEME, L. de O.
dc.contributor.authorFRANCO, M. M.
dc.contributor.authorFARIA, O. A. de
dc.contributor.authorCAETANO, A. R.
dc.contributor.authorSOUZA, J. G. de
dc.contributor.authorCARVALHEIRA, L. de R.
dc.contributor.authorSIQUEIRA FILHO, E. de
dc.contributor.authorDODE, M. A. N.
dc.date.accessioned2025-03-24T12:31:12Z-
dc.date.available2025-03-24T12:31:12Z-
dc.date.created2025-03-24
dc.date.issued2025
dc.identifier.citationScientific Reports, v. 15, 4839, 2025.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1174126-
dc.descriptionTo improve the quality of in vitro produced (IVP) embryos and reduce pregnancy losses, we proposed to reduce the components of the synthetic oviduct fluid (SOF) medium by 0% (SOF100), 50% (SOF50), and 75% (SOF25). First, embryos produced under these three treatments were evaluated for production, quality, lipid content, gene expression, and methylation patterns. The results indicated that all parameters analyzed were similar across all treatments (P > 0.05), suggesting that reducing media components does not affect embryo development and quality. Subsequently, we selected SOF25 for comparison with SOF100 in a commercial laboratory setting, evaluating embryo production, response to cryopreservation, gestation rate, and offspring birth. The data demonstrated that a 75% reduction in SOF medium components did not affect embryo development, quality, pregnancy rate, embryonic losses between 30 and 60 days, or birth rate (P > 0.05). To our knowledge, this is the first report on the pregnancy and birth rates of bovine blastocysts produced in media with nutrient concentrations as low as 25%. These results introduce novel cultural conditions that can be immediately incorporated into the IVF routine.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectBovine pregnancy rate
dc.subjectBovine birth rate
dc.subjectMedium nutrients reduction
dc.titleReduction of nutrients concentration in culture medium has no effect on bovine embryo production, pregnancy and birth rates.
dc.typeArtigo de periódico
dc.subject.nalthesaurusCulture media
riaa.ainfo.id1174126
riaa.ainfo.lastupdate2025-03-24
dc.identifier.doihttps://doi.org/10.1038/s41598-025-88864-x
dc.contributor.institutionLIGIANE DE OLIVEIRA LEME, FUNDAÇÃO DE AMPARO À PESQUISA DO DISTRITO FEDERAL – FAPDF
dc.contributor.institutionMAURICIO MACHAIM FRANCO, CENARGENeng
dc.contributor.institutionOTÁVIO AUGUSTO DE FARIA, UNIVERSIDADE DE BRASÍLIAeng
dc.contributor.institutionALEXANDRE RODRIGUES CAETANO, CENARGENeng
dc.contributor.institutionJULIANA GONÇALVES DE SOUZA, UNIVERSIDADE FEDERAL DE GOIÁSeng
dc.contributor.institutionLUCIANO DE REZENDE CARVALHEIRA, EMBRIOTECeng
dc.contributor.institutionEMIVALDO DE SIQUEIRA FILHO, EMBRIOTECeng
dc.contributor.institutionMARGOT ALVES NUNES DODE, CENARGEN.eng
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