Title:	Partial characterization of a bacteriocin-like inhibitory substance produced by Streptococcus equinus C6I9, a bovine rumen isolate.
Authors:	MAGALHÃES JÚNIOR, G. A. de FOCHAT, R. C. HUNGARO, H. M. RIBEIRO, J. B. LIMA, J. C. F. CARNEIRO, J. da C. VICENTINE, K. F. D. BARBOSA, A. A. T. PAIVA, A. D.
Affiliation:	GÉSSICA APARECIDA DE MAGALHÃES JÚNIOR, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; ROMÁRIO COSTA FOCHAT, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; HUMBERTO MOREIRA HÚNGARO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; JOAO BATISTA RIBEIRO, CNPGL; JUNIOR CESAR FERNANDES LIMA, CNPGL; JAILTON DA COSTA CARNEIRO, CNPGL; KARINA FERRAZZOLI DEVIENNI VICENTINE, UNIVERSIDADE FEDERAL DO TRIANGULO MINEIRO; ANA ANDREA TEIXEIRA BARBOSA, UNIVERSIDADE FEDERAL DE SERGIPE; ALINE DIAS PAIVA, UNIVERSIDADE FEDERAL DO TRIANGULO MINEIRO.
Date Issued:	2023
Citation:	Concilium, v. 23, n. 15, p. 78-96, 2023.
Description:	The present study aimed to evaluate the production and the factors that influence the antimicrobial activity of a bacteriocin-like inhibitory substance (BLIS) produced by Streptococcus equinus C6I9, a bovine rumen isolate. The antagonist activity was evaluated by the spot-on-lawn assay. Antimicrobial activity of the crude extracts obtained using NaCl acidic solution (pH 2, 100 mM) was evaluated along the bacterial growth and in growth medium containing different carbon and nitrogen sources, by agar diffusion method. Crude extract was evaluated regarding temperature stability, storage conditions, and pH sensitivity. S. equinus C619 inhibited both Gram-positive and Gram-negative strains. The crude extract was sensitive to proteinase K, and showed great stability to different temperatures and pH (2 to 12). The presence of meat extract (1.5 g/L) and sucrose (8 g/L) resulted in higher production of BLIS by S. equinus C6I9. The BLIS molecular mass was determined as approximately 3.5 kDa. The results obtained in this study evidenced the production of a broad spectrum activity, thermo- and pH stable BLIS by the ruminal isolate S. equinus C6I9, important features for its biotechnological application.
Thesagro:	Bovino Rúmen Bactéria Antibiótico Listeria Monocytogenes Microbiologia
NAL Thesaurus:	Streptococcus equinus
Keywords:	Atividade antimicrobiana Bacteriocina
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPGL)