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dc.contributor.authorFIGUEIREDO, J. E. F.
dc.contributor.authorDINIZ, G. de F.
dc.contributor.authorMARINS, M. S.
dc.contributor.authorSILVA, F. C.
dc.contributor.authorRIBEIRO, V. P.
dc.contributor.authorLANZA, F. E.
dc.contributor.authorOLIVEIRA-PAIVA, C. A.
dc.contributor.authorCRUZ-MAGALHÃES, V.
dc.date.accessioned2025-05-15T15:48:15Z-
dc.date.available2025-05-15T15:48:15Z-
dc.date.created2025-05-15
dc.date.issued2025
dc.identifier.citationFrontiers in Microbiology, v. 16, 1522136, 2025.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1175645-
dc.descriptionIntroduction: Bacillus velezensis is a ubiquitous bacterium with potent antifungal activity and a plant growth promoter. This study investigated the potential of B. velezensis CNPMS-22 as a biocontrol agent against phytopathogenic fungi under diverse experimental conditions and its potential as a plant growth promoter. Genome sequencing and analysis revealed putative genes involved in these traits. Methods: This research performed in vitro experiments to evaluate the CNPMS-22 antagonistic activity against 10 phytopathogenic fungi using dual culture in plate (DCP) and inverted sealed plate assay (ISP). Greenhouse and field tests evaluated the ability of CNPMS-22 to control Fusarium verticillioides in maize plants in vivo. The CNPMS-22 genome was sequenced using the Illumina HiSeq 4,000 platform, and genomic analysis also included manual procedures to identify genes of interest accurately. Results: CNPMS-22 showed antifungal activity in vitro against all fungi tested, with notable reductions in mycelial growth in both DCP and ISP experiments. In the ISP, volatile organic compounds (VOCs) produced by CNPMS-22 also altered the mycelium coloration of some fungi. Scanning electron microscopy revealed morphological alterations in the hyphae of F. verticillioides in contact with CNPMS-22, including twisted, wrinkled, and ruptured hyphae. Eight cluster candidates for synthesizing non-ribosomal lipopeptides and ribosomal genes for extracellular lytic enzymes, biofilm, VOCs, and other secondary metabolites with antifungal activity and plant growth promoters were identified by genomic analysis. The greenhouse and field experiments showed that seed treatment with CNPMS-22 reduced Fusarium symptoms in plants and increased maize productivity. Conclusion: Our findings highlight the CNPMS-22’s potential as bioinoculant for fungal disease control and plant growth with valuable implications for a sustainable crop productivity.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectBiofungicida
dc.subjectBioinoculante
dc.subjectGenome sequencing
dc.titleBacillus velezensis CNPMS-22 as biocontrol agent of pathogenic fungi and plant growth promoter.
dc.typeArtigo de periódico
dc.subject.thesagroFungo
dc.subject.thesagroMilho
dc.subject.thesagroRendimento
dc.subject.thesagroFungo Para Controle Biológico
dc.subject.nalthesaurusFungi
riaa.ainfo.id1175645
riaa.ainfo.lastupdate2025-05-15
dc.identifier.doihttps://doi.org/10.3389/fmicb.2025.1522136
dc.contributor.institutionJOSE EDSON FONTES FIGUEIREDO, CNPMS; GISELE DE FÁTIMA DIAS DINIZ; MIKAELY SOUSA MARINS; FELIPE CAMPOS SILVA, UNIVERSIDADE FEDERAL DE VIÇOSA; VITÓRIA PALHARES RIBEIRO; FABRÍCIO EUSTÁQUIO LANZA; CHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMS; VALTER CRUZ-MAGALHÃES.
Appears in Collections:Artigo em periódico indexado (CNPMS)

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