Title:	Comparison of post-thaw motility and in vitro fertility between ejaculated and epididymal semen, and seminal cfDNA characterization in Pantaneiro bulls.
Authors:	CARNEIRO, M. C. CASTRO, A. C. S. e SILVA, R. R. CHAVES, J. E. V. SILVA, V. A. O. CAPOBIANCO, N. E. QUEIROZ, P. J. B. MELO, L. de F. e BARBOSA, E. A. DODE, M. A. N. SPRÍCIGO, J. F. W.
Affiliation:	MARCOS COURA CARNEIRO, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); ALICE CAROLINE SOUZA E CASTRO, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); ROBERTA REIS SILVA, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); JOSÉ EDUARDO VIEIRA CHAVES, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); VENÂNCIO AUGUSTO OLIVEIRA SILVA, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); NATALIA ERNANDES CAPOBIANCO; PAULO JOSÉ BASTOS QUEIROZ, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); LEONARDO DE FRANÇA E MELO, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); ELEONORA ARAÚJO BARBOSA, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; MARGOT ALVES NUNES DODE, CENARGEN; JOSÉ FELIPE WARMLING SPRÍCIGO, UNIVERSIDADE FEDERAL DE GOIÁS (UFG).
Date Issued:	2025
Citation:	Biology, v. 14, 465, 2025.
Description:	This study investigated the motility and fertility of frozen-thawed semen from Pantaneiro bulls, comparing samples collected via ejaculation and directly from the epididymis. The aim was to evaluate the potential of epididymal semen for use in conservation programs by assessing its post-thaw motility and in vitro fertility. Semen from five bulls was analyzed for fresh quality, cell-free DNA (cfDNA) levels, and sperm performance after freezing. Results showed that epididymal semen had fewer defects and exhibited superior motility after thawing compared to ejaculated semen. Despite these differences, both semen sources were equally effective in fertilizing oocytes in vitro. The characterization of cfDNA levels adds valuable insights to the field of male fertility and supports efforts to conserve indigenous cattle breeds. This research contributes to improving semen preservation techniques and highlights the motility of epididymal semen as a reproductive resource in livestock breeding and conservation strategies.
Keywords:	Indigenous cattle Semen cryopreservation IVP Molecular marker
DOI:	https://doi.org/10.3390/biology14050465
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CENARGEN)