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dc.contributor.authorRABELO, M. B. de O.
dc.contributor.authorFREITAS, K. M.
dc.contributor.authorOLIVEIRA, L. C. de
dc.contributor.authorFRADE, A. C. M.
dc.contributor.authorS. FILHO, J. D. de
dc.contributor.authorHEIDEN, G.
dc.contributor.authorFERNANDES, G. W.
dc.contributor.authorSILVA, I. T.
dc.contributor.authorTEIXEIRA, M. M.
dc.contributor.authorBRAGA, F. C.
dc.contributor.authorPÁDUA, R. M.
dc.date.accessioned2025-07-01T19:54:24Z-
dc.date.available2025-07-01T19:54:24Z-
dc.date.created2025-07-01
dc.date.issued2025
dc.identifier.citationChemistry and Biodiversity, e202402824, 2025.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1176932-
dc.descriptionThe investigation of natural products is a valid strategy to identify compounds active against coronaviruses. We herein report the screening of Baccharis and Campovassouria plant species against the murine betacoronavirus murine hepatitis virus-3 (MHV-3), assays that can be carried out in BSL-2 facilities. These genera occur exclusively in the Americas and are a source of secondary metabolites with antiviral and anti-inflammatory activity. Campovassouria cruciata emerged as the most active species from the screening, and its phytochemical investigation afforded the discovery of a new isomer of leptocarpin, 3-epi-leptocapin (1), in addition to the sesquiterpenes lactones leptocarpin (2) and arturin (3), along with umbelliferone (4). The structures were elucidated by extensive mono- and two-dimensional 1H and 13C NMR data analysis. The antiviral activity of the isolated compounds was also assayed in Calu-3 cells infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). All compounds inhibited SARS-CoV-2 replication, with a reduction in viral load of approximately 1.7-2.0 log when tested at 7.5 µM. To evaluate potential anti-inflammatory activity, compounds were tested in A549 cells stimulated with IFN-γ and TNF-α. Both 3-epi-leptocarpin (1) and umbelliferone (4) inhibited interleukin-6 (IL-6) and IL-8 release significantly. Compound 1 reduced the production of reactive oxygen species (ROS) in A549 cells, as assessed by flow cytometry using DCFH-DA, whereas 4 exhibited ROS values seven-fold higher than the basal level. Our results highlight C. cruciata as a source of promising bioactive compounds, which deserve future investigations to explore their potential for the development of new therapeutic agents against inflammatory and viral diseases.
dc.language.isopor
dc.rightsopenAccess
dc.subjectLactonas sesquiterpênicas
dc.subjectSesquiterpene lactones
dc.subjectCoronavírus 2 da síndrome respiratória aguda grave
dc.titleActivity of Sesquiterpene Lactones and Umbelliferone From <i>Campovassouria cruciata</i> on SARS‐CoV‐2 Replication and on the Release of Pro‐Inflammatory Cytokines in Lung Cells
dc.typeArtigo de periódico
dc.subject.thesagroAntiinflamatório
dc.subject.nalthesaurusAntiviral agents
dc.subject.nalthesaurusAnti-inflammatory agents
dc.subject.nalthesaurusSevere acute respiratory syndrome coronavirus
riaa.ainfo.id1176932
riaa.ainfo.lastupdate2025-07-01
dc.identifier.doi10.1002/cbdv.202402824
dc.contributor.institutionMARIA BEATRIZ DE O. RABELO, UNIVERSIDADE FEDERAL DE MINAS GERAIS; KÁTIA M. FREITAS, UNIVERSIDADE FEDERAL DE MINAS GERAIS; LEONARDO C. DE OLIVEIRA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; ALESSANDRA C. M. FRADE, UNIVERSIDADE FEDERAL DE MINAS GERAIS; JOSÉ D. DE S. FILHO, UNIVERSIDADE FEDERAL DE OURO PRETO; GUSTAVO HEIDEN, CPACT; GERALDO W. FERNANDES, UNIVERSIDADE FEDERAL DE MINAS GERAIS; IZABELLA THAÍS SILVA, UNIVERSIDADE FEDERAL DE SANTA CATARINA; MAURO MARTINS TEIXEIRA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; FERNÃO C. BRAGA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; RODRIGO M. PÁDUA, UNIVERSIDADE FEDERAL DE MINAS GERAIS.
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