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dc.contributor.authorMASCARIN, G. M.
dc.contributor.authorSHRESTHA, S.
dc.contributor.authorCORTES, M. V. de C. B.
dc.contributor.authorRAMIREZ, J. L.
dc.contributor.authorDUNLAP, C. A.
dc.contributor.authorCOLEMAN, J. J.
dc.date.accessioned2025-07-02T10:48:06Z-
dc.date.available2025-07-02T10:48:06Z-
dc.date.created2025-07-01
dc.date.issued2024
dc.identifier.citationFungal Biology and Biotechnology, v. 11, article 21, 2024.
dc.identifier.issn2054-3085
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1176935-
dc.descriptionAbstract: Biocontrol agents play a pivotal role in managing pests and contribute to sustainable agriculture. Recent advancements in genetic engineering can facilitate the development of entomopathogenic fungi with desired traits to enhance biocontrol efficacy. In this study, a CRISPR-Cas9 ribonucleoprotein system was utilized to genetically improve the virulence of Beauveria bassiana, a broad-spectrum insect pathogen used in biocontrol of arthropod pests worldwide. CRISPR-Cas9-based disruption of the transcription factor-encoding gene Bbsmr1 led to derepression of the oosporein biosynthetic gene cluster resulting in overproduction of the red-pigmented dibenzoquinone oosporein involved in host immune evasion, thus increasing fungal virulence. Mutants defective for Bbsmr1 displayed a remarkable enhanced insecticidal activity by reducing lethal times and concentrations, while concomitantly presenting negligible or minor pleiotropic effects. In addition, these mutants displayed faster germination on the insect cuticle which correlated with higher density of free-floating blastospores in the hemolymph and accelerated mortality of the host. These findings emphasize the utility of genetic engineering in developing enhanced fungal biocontrol agents with customized phenotypic traits, and provide an efficient and versatile genetic transformation tool for application in other beneficial entomopathogenic fungi.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectGenome editing
dc.subjectGeneticin selectable marker
dc.titleCRISPR-Cas9-mediated enhancement of Beauveria bassiana virulence with overproduction of oosporein.
dc.typeArtigo de periódico
dc.subject.thesagroBeauveria Bassiana
dc.subject.thesagroFungo Para Controle Biológico
dc.subject.thesagroEngenharia Genética
dc.subject.nalthesaurusSecondary metabolites
dc.subject.nalthesaurusBlastospores
dc.subject.nalthesaurusRibonucleoproteins
dc.subject.nalthesaurusHomologous recombination
riaa.ainfo.id1176935
riaa.ainfo.lastupdate2025-07-01
dc.identifier.doihttps://doi.org/10.1186/s40694-024-00190-5
dc.contributor.institutionGABRIEL MOURA MASCARIN, CNPMA; SOMRAJ SHRESTHA, AUBURN UNIVERSITY; MARCIO VINICIUS DE CARVALHO BARROS CORTES, CNPAF; JOSE LUIS RAMIREZ, UNITED STATES DEPARTMENT OF AGRICULTURE; CHRISTOPHER A DUNLAP, UNITED STATES DEPARTMENT OF AGRICULTURE; JEFFREY J COLEMAN, AUBURN UNIVERSITY.
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