Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1178528
Full metadata record
DC FieldValueLanguage
dc.contributor.authorMORIYA, J. C. K.eng
dc.contributor.authorSUNIGA, P. A. P.eng
dc.contributor.authorARAÚJO, A. C. L.eng
dc.contributor.authorSANTOS, M. G. doseng
dc.contributor.authorRIEGER, J. S. G.eng
dc.contributor.authorMANTOVANI, C.eng
dc.contributor.authorJARDIM, R.eng
dc.contributor.authorSILVA, M. R.eng
dc.contributor.authorARAUJO, F. R.eng
dc.contributor.authorSANTOS, L. R. doseng
dc.date.accessioned2025-09-04T11:48:47Z-
dc.date.available2025-09-04T11:48:47Z-
dc.date.created2025-09-04
dc.date.issued2025
dc.identifier.citationPathogens, v. 14, 766, 2025.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1178528-
dc.descriptionGlanders is a highly contagious and often fatal zoonotic disease of equids caused by Burkholderia mallei, a pathogen of significant concern due to its potential for bioterrorism. In Brazil, glanders remains endemic, particularly among working equids in the Northeast region. Diagnostic confirmation typically involves serology, culture, and polymerase chain reaction (PCR), although false-negative PCR results have been increasingly reported. This study aimed to evaluate the diagnostic performance and analytical sensitivity of four B. mallei-specific PCR primer sets using samples from 30 seropositive equids. Microbiological cultures were obtained from various organs and swabs, followed by PCR targeting four genomic regions: fliP-IS407A(a), fliP-IS407A(b), Burk457, and Bm17. All animals were confirmed positive for B. mallei via culture, but PCR detection rates varied significantly across primer sets. The fliP-IS407A(b) primer set showed the highest sensitivity, detecting 86% of samples, while the WOAH-recommended fliP-IS407A(a) set had the lowest performance (13.4%). Analytical sensitivity assays confirmed that fliP-IS407A(b) and Bm17 primers detected DNA concentrations as low as 0.007 ng, outperforming the others. These findings suggest that certain widely used primer sets may lack sufficient sensitivity for reliable detection of B. mallei, especially in chronically infected animals with low bacterial loads. The study underscores the need for ongoing validation of molecular diagnostics to improve the detection and control of glanders in endemic regions.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectEquídeo
dc.titleDetection of Burkholderia mallei in microbiological culture: a comparative analysis of PCR primer sets.
dc.typeArtigo de periódico
dc.subject.thesagroPatógeno
dc.subject.thesagroBactéria
dc.subject.thesagroDoença Animal
dc.subject.thesagroCavalo
dc.subject.thesagroMula
dc.subject.thesagroZoonose
riaa.ainfo.id1178528
riaa.ainfo.lastupdate2025-09-04
dc.identifier.doihttps://doi.org/10.3390/pathogens14080766
dc.contributor.institutionJÉSSICA CRISTINE K. MORIYAeng
dc.contributor.institutionPAULA ADAS P. SUNIGAeng
dc.contributor.institutionANA CLARA L. ARAÚJO, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SULeng
dc.contributor.institutionMARIA GORETTI DOS SANTOS, CNPGCeng
dc.contributor.institutionJULIANA S. G. RIEGEReng
dc.contributor.institutionCYNTHIA MANTOVANIeng
dc.contributor.institutionRODRIGO JARDIM, INSTITUTO OSWALDO CRUZeng
dc.contributor.institutionMARCIO ROBERTO SILVA, CNPGLeng
dc.contributor.institutionFLABIO RIBEIRO DE ARAUJO, CNPGCeng
dc.contributor.institutionLENITA RAMIRES DOS SANTOS, CNPGC.eng
Appears in Collections:Artigo em periódico indexado (CNPGL)

Files in This Item:
File Description SizeFormat 
Detection-of-Burkholderia-mallei.pdf1.65 MBAdobe PDFThumbnail
View/Open
Show simple item record

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace