Title:	Comparative analysis of structuralchromosome variability and heterochromatin localization in Passiflora L. (Passifloraceae).
Authors:	SANTOS, K. R. G. dos MARÇAL, K. L. G. NASCIMENTO, T. L. dos MELO, N. F. de
Affiliation:	KEDMA RAISSA G. DOS SANTOS, UNIVERSIDADE ESTADUAL DE FEIRA DE SANTANA; KANANDA LAIRA G. MARÇAL, UNIVERSIDADE ESTADUAL DE FEIRA DE SANTANA; TIAGO L. DO NASCIMENTO, FUNDAÇÃO DE AMPARO À CIÊNCIA E TECNOLOGIA DE PERNAMBUCO; NATONIEL FRANKLIN DE MELO, CPATSA.
Date Issued:	2025
Citation:	Anais da Academia Brasileira de Ciências, v. 97, n. 4, 2025.
Description:	Passiflora is the most diverse genus of the Passifloraceae family, and holds great economic importance. However, cytogenetic information is still limited for most species. This study aimed to analyze the karyotypes of nine Passiflora species, to determine the chromosome number and the distribution of heterochromatic regions by double staining with CMA/DAPI and by fluorescence in situ hybridization (FISH) with 35S and 5S rDNA probes. Eight species presented a karyotype with 2n=18 chromosomes, and in contrast, P. foetida was the only species to exhibit a karyotype with 2n=20. Double staining with CMA and DAPI revealed distinct staining patterns, with four CMA+ blocks located in the terminal regions of the chromosomes of eight species, except in P. foetida, where six CMA+ blocks were observed in the proximal regions. FISH identified four to six 35S rDNA sites in the terminal regions of the chromosomes in P. alata and P. edmundoi, and six sites in interstitial region in P. foetida. In these same species, two 5S rDNA sites were observed, and in P. foetida four sites in the subterminal regions. The observed chromosomal variations suggest significant genetic diversity in the genus, valuable data for studies of diversity, conservation and genetic improvement. Key words: karyotype, CMA, DAPI, FISH, passion fruit.
Thesagro:	Maracujá Melhoramento Genético Vegetal Cariótipo
NAL Thesaurus:	Passiflora Passion fruits
Keywords:	CMA DAPI
DOI:	https://doi.org/10.1590/0001-3765202520250272
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPATSA)