Title:	Cleavage of mangiferin by Acinetobacter SM902 isolated from Mangifera indica rhizosphere: production of norathyriol and other phenolic compounds.
Authors:	SILVA, G. O. ALVES NETO, M. L. SILVA, F. L. DA TREVISAN, M. T. S. BRITO, E. S. de MARQUES, S. P. D. CESAR, C. L. MELO, V. M. M. ZAMPIERI, D.
Affiliation:	GABRIELLY O. SILVA, UNIVERSIDADE FEDERAL DO CEARÁ; MANOEL L. ALVES-NETO, UNIVERSIDADE FEDERAL DO CEARÁ; FRANCISCO L. DA SILVA, UNIVERSIDADE FEDERAL DO CEARÁ; MARIA T. S. TREVISAN, UNIVERSIDADE FEDERAL DO CEARÁ; EDY SOUSA DE BRITO, CNAT; SAMUEL P. D. MARQUES, UNIVERSIDADE FEDERAL DO CEARÁ; CARLOS L. CESAR, UNIVERSIDADE FEDERAL DO CEARÁ; VÂNIA M. M. MELO, UNIVERSIDADE FEDERAL DO CEARÁ; DAVILA ZAMPIERI, UNIVERSIDADE FEDERAL DO CEARÁ.
Date Issued:	2025
Citation:	Bioorganic Chemistry, n. 166, 2025, 109099.
Description:	Norathyriol is one of the xanthone aglycone synthesized through the shikimate pathway, and it serves as a precursor of mangiferin in plant metabolism. In microbial metabolism, however, it can appear as a product of mangiferin degradation. Norathyriol plays an importante role as an anti-inflammatory, anticancer, and antioxidant agent. In comparison with chemical synthesis, the use of microorganisms capable to metabolize mangiferin has advantages, since it does not require strong acids or phenolic reagents. The aim of this study was to produce norathyriol from mangiferin using a bacterial strain SM902 isolated from the rhizosphere of Mangifera indica (mango). The strain was identified, and its ability to degrade mangiferin was analyzed. Rhizosphere and bulk soil samples of M. indica were inoculated into synthetic medium containing mangiferin as the only carbon source, and the bacterial isolates were screened. Strain SM902 was identified as Acinetobacter sp. through 16S rRNA gene sequencing. Photoluminescence excitation analysis confirmed the production of norathyriol as a biotransformation product. In addition, the SM902 extract was examined by ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry in MSE mode (UPLC-QTOF- MSE), allowing the characterization of monogalloyl-glucose, homomangiferin, tetrahydroxyxanthone-C-hexoside dimer and its isomers as additional products from mangiferin biotransformation. This is the first report of an aerobic Gram-negative bacterium able to metabolize mangiferin, producing norathyriol and other phenolic compounds with potential pharmacological relevance. Further studies are still necessary to identify the metabolic pathway involved in this process.
Thesagro:	Bactéria
NAL Thesaurus:	Biotransformation Photoluminescence
Keywords:	C deglycosylation UPLC MS
DOI:	10.1016/j.bioorg.2025.109099
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNAT)