Title:	Action of melatonin in bovine embryo culture under high oxygen tension in blastocyst production.
Authors:	OLIVEIRA, I. R. dos S. RODRIGUES, F. L. FARIA, L. C. MESQUITA, A. Q. de RODRIGUES, M. T. de O. MARTINS, G. A. M. M. MELLO, V. C. FONSECA, M. J. P. MARTINS, C. F. BÁO, S. N.
Affiliation:	ISABELLA RODRIGUES DOS SANTOS OLIVEIRA, UNIVERSIDADE DE BRASÍLIA; FABIANA LIMA RODRIGUES, CENTRO UNIVERSITÁRIO ICESP; LUCAS COSTA DE FARIA, UNIVERSIDADE DE BRASÍLIA; ADRIANO QUEIROZ DE MESQUITA, CPAC; MARIA TEREZA DE OLIVEIRA RODRIGUES, UNIVERSIDADE DE BRASÍLIA; GABRIEL ANTÔNIO M. MEDEIROS MARTINS, CENTRO UNIVERSITÁRIO DE BRASÍLIA; VICTOR CARLOS MELLO, UNIVERSIDADE DE BRASÍLIA; MÁRCIO JOSÉ POÇAS FONSECA, UNIVERSIDADE DE BRASÍLIA; CARLOS FREDERICO MARTINS, CPAC; SÔNIA NAIR BÁO, UNIVERSIDADE DE BRASÍLIA.
Date Issued:	2025
Citation:	Animal Reproduction, v. 22, n. 3, 2025.
Description:	Melatonin (Mel) is an antioxidant that modulates the microenvironment where oxidative stress is one of the main challenges to embryonic viability. In this context, this study evaluated the impact of supplementation with melatonin at 10⁻⁹ M during the in vitro culture of bovine embryos under different oxygen tensions. After in vitro fertilization, bovine zygotes were cultured in four experimental groups: SOF with and without melatonin under high oxygen tension (high O₂ with Mel/without Mel) and SOF with and without melatonin under low oxygen tension (low O₂ with Mel/without Mel). On day 7, blastocyst rate, gene expression related of oxidative stress and embryo quality, mitochondrial activity (MitoTracker), and cytoplasmic lipid accumulation (Bodipy) were evaluated.
Thesagro:	Bovino Cultura de Embrião
NAL Thesaurus:	Blastocyst Embryo culture In vitro fertilization Melatonin Oxidative stress
Notes:	Edição dos Proceedings 38th Annual Meeting of the Brazilian Embryo Technology Society (SBTE), 2025.
Type of Material:	Resumo em anais e proceedings
Access:	openAccess
Appears in Collections:	Resumo em anais de congresso (CPAC)