Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1180582
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dc.contributor.authorRODRIGUES, F. L.
dc.contributor.authorOLIVEIRA, I. R. dos S.
dc.contributor.authorMELO, J. C. de
dc.contributor.authorSILVA, J. R. S. da
dc.contributor.authorFERREIRA, I. C.
dc.contributor.authorMARTINS, C. F.
dc.date.accessioned2025-10-24T14:48:35Z-
dc.date.available2025-10-24T14:48:35Z-
dc.date.created2025-10-24
dc.date.issued2025
dc.identifier.citationAnimal Reproduction, v. 22, n. 3, 2025.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1180582-
dc.descriptionThe establishment of a germplasm bank using somatic cells is an important step prior to the use of genetic material in the bovine cloning process. Considering that each freezing curve temperature has specific implications for cell viability and functionality, the objective of this study was to evaluate the effect of three freezing protocols for fibroblasts from a high-genetic-merit dairy Sindi bull intended for cloning: 1. Pre-freezing at -80°C for 24 hours followed by immersion in liquid nitrogen (LN2); 2. Pre-freezing at -20°C for 24 hours followed by immersion in LN2; 3. Cooling to 5°C for 1 hour, gradual freezing in LN2 vapor for 20 minutes, and storage in LN2.
dc.language.isoeng
dc.rightsopenAccess
dc.titleEffect of different protocols for cryopreservation of skin fibroblasts from Sindi dairy bull.
dc.typeResumo em anais e proceedings
dc.subject.thesagroBanco de Germoplasma
dc.subject.thesagroBovino
dc.subject.thesagroGado Sindi
dc.subject.thesagroClonagem
dc.subject.thesagroCriopreservação
dc.subject.thesagroTouro
dc.subject.nalthesaurusCryopreservation
dc.subject.nalthesaurusFibroblasts
dc.subject.nalthesaurusDairy bulls
dc.subject.nalthesaurusSkin (animal)
dc.subject.nalthesaurusSomatic cells
dc.description.notesEdição dos Proceedings 38th Annual Meeting of the Brazilian Embryo Technology Society (SBTE), 2025.
riaa.ainfo.id1180582
riaa.ainfo.lastupdate2025-10-24
dc.contributor.institutionFABIANA LIMA RODRIGUES, CENTRO UNIVERSITÁRIO ICESP; ISABELLA RODRIGUES DOS SANTOS OLIVEIRA, UNIVERSIDADE DE BRASÍLIA; JULIA CARVALHO DE MELO, UNIVERSIDADE DE BRASÍLIA; JOSÉ RICARDO SOBRAL DA SILVA, CENTRO UNIVERSITÁRIO DO PLANALTO CENTRAL; ISABEL CRISTINA FERREIRA, CPAC; CARLOS FREDERICO MARTINS, CPAC.
Appears in Collections:Resumo em anais de congresso (CPAC)

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