Title:	Dietary modulation of the rumen microbiome drives the expression of metabolic and methanogenic pathways in Bos indicus.
Authors:	SILVA, J. V. da CONTEVILLE, L. C. BRUSCADIN, J. J. CARDOSO, T. F. PORTO, T. OLIVEIRA, P. S. N. de ZERLOTINI NETO, A. MEDEIROS, S. R. de MOURÃO, G. B. COUTINHO, L. L. PALHARES, J. C. P. BERNDT, A. GUAN, L. L. ANDRADE, B. G. N. REGITANO, L. C. de A.
Affiliation:	JULIANA VIRGINIO DA SILVA, UNIVERSIDADE FEDERAL DE SÃO CARLOS LILIANE COSTA CONTEVILLE, FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO JENNIFER JESSICA BRUSCADIN, UNIVERSIDADE FEDERAL DE SÃO CARLOS TAINÃ FIGUEIREDO CARDOSO THANNY PORTO, UNIVERSIDADE FEDERAL DE SÃO CARLOS PRISCILA SILVA NEUBERN DE OLIVEIRA, UNIVERSIDADE FEDERAL DE SÃO CARLOS ADHEMAR ZERLOTINI NETO, CNPTIA SERGIO RAPOSO DE MEDEIROS, CPPSE GERSON BARRETO MOURÃO, UNIVERSIDADE DE SÃO PAULO LUIZ LEHMANN COUTINHO, UNIVERSIDADE DE SÃO PAULO JULIO CESAR PASCALE PALHARES, CPPSE ALEXANDRE BERNDT, CPPSE LE LUO GUAN, UNIVERSITY OF BRITISH COLUMBIA BRUNO GABRIEL NASCIMENTO ANDRADE, MUNSTER TECHNOLOGICAL UNIVERSITY LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.
Date Issued:	2025
Citation:	mSphere, v. 10, n. 10, e00535-25, Oct. 2025.
Description:	ABSTRACT. Diet influences ruminal methane emissions by modulating the compositionand activity of the rumen microbiome. However, how diet shapes the functional capacityof the rumen microbiome in Nelore cattle (Bos indicus), a key tropical beef breed, remainsunclear. This study used metatranscriptomics to investigate how dietary supplementation with agro-industrial by-products affects the active rumen microbiome and itsassociation with residual methane emissions. Rumen samples from 50 Nelore cattle fedeither a conventional or by-product-based diet revealed that the active microbiome wasdominated by bacteria (88.4% ± 3.16%) and archaea (11.6% ± 3.16%), with no significanttaxonomic differences between diets. Despite this, functional profiling identified genesfrom 193 pathways and 3,512 gene families, with distinct metabolic signatures betweendiets. Specifically, six pathways and 87 gene families were unique to the conventionaldiet, while seven pathways and 210 gene families were unique to the by-product diet.The associations between gene families enriched under each diet with residual methaneemission revealed that the expression of two gene families exhibited negative correlations, while five were positively correlated with methane emission under conventionaldiet. In the by-product diet, we identified five gene families positively associated withmethane emissions and 14 negatively associated. These results demonstrate that dietalters rumen microbial functions with methane mitigation potential, without affectingtaxonomic composition.
Thesagro:	Gado de Corte Bos Indicus
NAL Thesaurus:	Beef cattle Citrus pulp Greenhouse gases Gene expression Peanut meal
Keywords:	Polpa cítrica Mudanças induzidas pela dieta Gases do efeito estufa Metatranscriptoma Expressão gênica do microbioma Farinha de amendoim Emissão de metano residual Fermentação ruminal Diet-induced changes Metatranscriptome Microbiome gene expression Residual methane emission Ruminal fermentation
ISSN:	2379-5042
DOI:	https://doi.org/10.1128/msphere.00535-25
Notes:	Na publicação: Adhemar Zerlotini.
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPTIA)