Title:	Embryogenic cultures and somatic embryos development from mature seeds of jabuticaba (Plinia cauliflora (Mart.) Kausel).
Authors:	OLIVEIRA, F. L. R. SANT’ANNA-SANTOS, B. F. FRAGA, H. P. DEGENHARDT, J. QUOIRIN, M.
Affiliation:	FABRÍCIA LORRANE R. OLIVEIRA, UNIVERSIDADE FEDERAL DO PARANÁ BRUNO FRANCISCO SANT’ANNA-SANTOS, UNIVERSIDADE FEDERAL DO PARANÁ HUGO P.F. FRAGA, UNIVERSIDADE FEDERAL DO PARANÁ JULIANA DEGENHARDT GOLDBACH, CNPF MARGUERITE QUOIRIN, UNIVERSIDADE FEDERAL DO PARANÁ.
Date Issued:	2022
Citation:	Anais da Academia Brasileira de Ciências, v. 94, n. 4, 2022.
Description:	Plinia cauliflora is an important Brazilian species that produces highly appreciated fruits, with a great potential of commercialization. However, the high cost of seedlings is a bottleneck for the expansion of commercial orchards. The present study aimed to investigate somatic embryogenesis as a propagation method for P. cauliflora using seeds as explants. To induce embryogenic mass (EM) and somatic embryo (SE) development we evaluated the supplementation of culture medium with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), combined or not with activated charcoal (AC). For the embryo maturation, we investigated the effects of AC, polyethylene glycol (PEG), Gelzan®, 6-benzylaminopurine and gibberellin supplementation. For the EM induction, the best results were obtained in MS culture medium supplemented with 300 μM 2,4-D and 1 g L-1 AC. During the first maturation phase, the supplementation of 30 g L-1 PEG improved the somatic embryo formation at the torpedo and cotyledonary stages, whereas the maturation treatments did not result in the conversion of the embryos into plantlets. The anatomical analysis showed that the 2,4-D presence for 60 days may have been deleterious for embryonic development. These results represent the first report of P. cauliflora somatic embryogenesis and its feasibility for mass propagation.
Thesagro:	Myrtaceae Micropropagação Jabuticaba Embriogénese
NAL Thesaurus:	Micropropagation Osmotic stress Plinia cauliflora Somatic embryogenesis
Keywords:	Ácido diclorofenoxiacético Auxin Estresse osmótico
ISSN:	1678-2690
DOI:	https://doi.org/10.1590/0001-3765202220201073
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPF)