Por favor, use este identificador para citar o enlazar este ítem: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1181561
Registro completo de metadatos
Campo DCValorLengua/Idioma
dc.contributor.authorAMARAL, H. B. S.
dc.contributor.authorSILVEIRA, M. M.
dc.contributor.authorNICOLÁS, A. C. C. V.
dc.contributor.authorPIMENTA, L. K. L.
dc.contributor.authorCHAVES, J. E. V.
dc.contributor.authorCAETANO, A. R.
dc.contributor.authorFRANCO, M. M.
dc.contributor.authorDODE, M. A. N.
dc.date.accessioned2025-11-17T12:53:18Z-
dc.date.available2025-11-17T12:53:18Z-
dc.date.created2025-11-17
dc.date.issued2025
dc.identifier.citationAntioxidants, v. 14, n. 11, 1322, 2025.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1181561-
dc.descriptionThis study aimed to evaluate the effects of melatonin supplementation during bovine in vitro embryo production (IVEP) on embryonic development and quality, oxidative stress, lipid metabolism, mitochondrial activity, gene expression, DNA methylation patterns, and cryotolerance. Four treatments were tested: control (without melatonin), melatonin at maturation (IVM + Mlt), culture (IVC + Mlt), and both treatments (IMV/IVC + Mlt). Melatonin significantly improved blastocyst rate and developmental kinetics on D7, reduced ROS and intracellular lipid levels, and increased mitochondrial activity. The most significant effects were observed in the IVC + Mlt group. Melatonin modulated antioxidant (SOD1, Cat, and GSS) and epigenetic (TET1, TET3, and DNMT3A) genes, and although it did not alter lipid gene expression, it reduced lipid content. Methylation analysis showed hypomethylation patterns in repetitive regions (Satellite I and LINE-1), which were even more pronounced in the melatonin-treated groups. However, no significant differences were observed between treatments in terms of cryotolerance or apoptosis rates. These findings suggest that melatonin exerts positive multifactorial effects, regardless of the supplementation stage. In particular, its addition during the IVC phase appears to provide greater benefits to embryos by improving their quality.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectBovine
dc.titleMelatonin improves bovine embryo production and quality via antioxidant, metabolic, and epigenetic pathways.
dc.typeArtigo de periódico
dc.subject.nalthesaurusOxidative stress
dc.subject.nalthesaurusGene expression
dc.subject.nalthesaurusMethylation
dc.subject.nalthesaurusCryopreservation
riaa.ainfo.id1181561
riaa.ainfo.lastupdate2025-11-17
dc.contributor.institutionHALLYA BEATRIZ SOUSA AMARAL, UNIVERSITY OF BRASILIA
dc.contributor.institutionMÁRCIA MARQUES SILVEIRA, FEDERAL DISTRICT RESEARCH SUPPORT FOUNDATION—FAP DFeng
dc.contributor.institutionANA CAROLINE CHAVES VALL NICOLÁS, UNIVERSITY OF BRASILIAeng
dc.contributor.institutionLARYSSA KETELYN LIMA PIMENTA, UNIVERSITY OF BRASILIAeng
dc.contributor.institutionJOSÉ EDUARDO VIEIRA CHAVES, FEDERAL UNIVERSITY OF GOIÁS—UFGeng
dc.contributor.institutionALEXANDRE RODRIGUES CAETANO, CENARGENeng
dc.contributor.institutionMAURICIO MACHAIM FRANCO, CENARGENeng
dc.contributor.institutionMARGOT ALVES NUNES DODE, CENARGEN.eng
Aparece en las colecciones:Artigo em periódico indexado (CENARGEN)

Ficheros en este ítem:
Fichero Descripción TamañoFormato 
antioxidants-14-01322-v2.pdf2,61 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro sencillo del ítem

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace