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dc.contributor.authorCAMARGO, L. S. de A.
dc.contributor.authorVIEIRA, C. D.
dc.contributor.authorPEREIRA, H. P.
dc.contributor.authorLEMOS, D. R. de
dc.contributor.authorSOUZA, E. D. de
dc.contributor.authorREIS, D. R. de L.
dc.contributor.authorSARAIVA, N. Z.
dc.contributor.authorOLIVEIRA, C. S.
dc.contributor.authorQUINTAO, C. C. R.
dc.contributor.authorSIQUEIRA, L. G. B.
dc.date.accessioned2025-11-24T18:49:31Z-
dc.date.available2025-11-24T18:49:31Z-
dc.date.created2025-11-24
dc.date.issued2025
dc.identifier.citationAnimal Reproduction, v. 22, n. 3, 2025.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1181807-
dc.descriptionCRISPR/Cas9 technology is a valuable tool for cattle precision breeding. The guide (sgRNA) and Cas9 can be injected into zygote’s cytoplasm; however, it is timing consuming and can impair embryo viability. We have shown that electroporation can be used to deliver sgRNA and Cas9 effectively, promoting gene editing in bovine embryos without affecting their in vitro viability (Camargo et al. 2020 Front Genet 11: 570069). In this study, our objective was to assess whether in vitro-fertilized and electroporated embryos could successfully generate gene-edited Angus calves.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectFertilizacao in vitro
dc.subjectEletroporação
dc.titleBirth of prolactin receptor-edited Angus calves produced through the electroporation of in vitrofertilized zygotes using CRISPR/Cas9 technology.
dc.typeResumo em anais e proceedings
dc.subject.thesagroBovino
dc.subject.thesagroReprodução Animal
dc.subject.thesagroEmbrião Animal
dc.subject.thesagroMelhoramento Genético Animal
dc.description.notesEdição dos resumos da 38th Annual Meeting of the Brazilian Embryo Technology Society, 2025, Costa do Sauípe, BA.
riaa.ainfo.id1181807
riaa.ainfo.lastupdate2025-11-24
dc.contributor.institutionLUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; CAROLINA DAVID VIEIRA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; HYAGO PASSE PEREIRA; DIANA RANGEL DE LEMOS, UNIVERSIDADE FEDERAL DE VIÇOSA; ELIZA DINIZ DE SOUZA; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; NAIARA ZOCCAL SARAIVA, CNPGL; CLARA SLADE OLIVEIRA, CNPGL; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL.
Aparece nas coleções:Resumo em anais de congresso (CNPGL)

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