Title:	Functional adaptations of the rhizosphere microbiome for drought-tolerance promotion in common bean.
Authors:	SILVA, A. V. R. da CUNHA, I. de C. M. PELLEGRINETTI, T. A. BOLETA, E. H. M. ZAGATTO, L. F. G. ZAGATTO, S. dos S. S. NISHISAKA, C. S. MAFRA, T. M. L. PATREZE, C. M. CUSTER, G. F. DINI-ANDREOTE, F. MENDES, R. TSAI, S. M. MENDES, L. W.
Affiliation:	ANA VITÓRIA REINA DA SILVA, UNIVERSIDADE DE SÃO PAULO; IZADORA DE CÁSSIA MESQUITA CUNHA, UNIVERSIDADE DE SÃO PAULO; THIERRY ALEXANDRE PELLEGRINETTI, UNIVERSIDADE DE SÃO PAULO; EDUARDO HENRIQUE MARCANDALLI BOLETA, UNIVERSIDADE DE SÃO PAULO; LUIS FELIPE GUANDALIN ZAGATTO, NETHERLANDS INSTITUTE OF ECOLOGY; SOLANGE DOS SANTOS SILVA ZAGATTO, UNIVERSIDADE DE SÃO PAULO; CAROLINE SAYURI NISHISAKA, UNIVERSIDADE DE SÃO PAULO; TERESA MARIA LORIZOLLA MAFRA, UNIVERSIDADE DE SÃO PAULO; CAMILA MAISTRO PATREZE, UNIVERSIDADE FEDERAL DO ESTADO DO RIO DE JANEIRO; GORDON F. CUSTER, UNIVERSITY OF MARYLAND EASTERN SHORE; FRANCISCO DINI-ANDREOTE, UNIVERSIDADE DE SÃO PAULO; RODRIGO MENDES, CNPMA; SIU MUI TSAI, UNIVERSIDADE DE SÃO PAULO; LUCAS WILLIAM MENDES, UNIVERSIDADE DE SÃO PAULO.
Date Issued:	2025
Citation:	Plant Stress, v. 16, article 100860, 2025.
Description:	Abstract: Drought stress threatens global food security, highlighting the need for resilient crops. Harnessing rhizosphere microorganisms can improve plant performance in harsh conditions. Here, we investigated the rhizosphere microbiomes of drought-tolerant (BAT477, SEA5) and susceptible (IAC Milênio, IAC-Carioca 80SH) common bean cultivars (Phaseolus vulgaris L.) under contrasting water regimes in mesocosm experiments to assess microbiome functional modulation under drought. Analysis of plant growth, physiological responses, nutrient dynamics, and rhizosphere microbial functional diversity revealed that drought-tolerant cultivars exhibited greater water management, minimal growth reductions, and enrichment of beneficial microbial functions, including genes linked to drought tolerance. Notably, drought stress triggered differential abundance in 1864 microbial genes, highlighting a robust functional shift. Specifically, drought-tolerant cultivars showed an enrichment of genes related to osmotic response, photosynthetic efficiency (82–87 % reduction in photosynthesis in susceptible cultivars), oxidative stress mitigation, and osmoprotectant production, whereas susceptible cultivars relied more on genes associated with DNA repair and antioxidant defense, indicating a reactive rather than proactive stress response. Additionally, the rhizosphere microbiomes of drought-tolerant cultivars were enriched in functions related to biofilm formation, dormancy survival, and oxidative stress resistance. These cultivars also maintained higher photosynthetic activity and transpiration rates with more stable stomatal conductance. Upon rehydration, they partially restored physiological functions (e.g., 48–57 % recovery in photosynthesis), further demonstrating microbiome-conferred resilience. These findings underscore the potential of plant-microbiome interactions in adapting to water stress, suggesting that microbiome selection could be a promising strategy for developing drought-resilient crops and advancing sustainable agricultural practices.
Thesagro:	Feijão Resistência a Seca População Microbiana Rizosfera
NAL Thesaurus:	Beans Abiotic stress Microbiome Water stress
Keywords:	Abiotic stress Plant-microbe interactions
ISSN:	2667-064X
DOI:	https://doi.org/10.1016/j.stress.2025.100860
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPMA)