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| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.contributor.author | LIMA, E. R. | |
| dc.contributor.author | SEVILHANO, T. C. A. | |
| dc.contributor.author | FEITOSA, T. C. | |
| dc.contributor.author | OLIVEIRA, J. E. | |
| dc.contributor.author | SUZUKI, M. F. | |
| dc.contributor.author | TORATI, L. S. | |
| dc.contributor.author | BARTOLINI, P. | |
| dc.contributor.author | PERONI, C. N. | |
| dc.date.accessioned | 2025-11-27T13:48:56Z | - |
| dc.date.available | 2025-11-27T13:48:56Z | - |
| dc.date.created | 2025-11-27 | |
| dc.date.issued | 2025 | |
| dc.identifier.citation | Fishes, v. 10, n. 12, p. 607, 2025. | |
| dc.identifier.uri | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1181967 | - |
| dc.description | The cDNA sequences of the common gonadotrophic hormone α-subunit (ag-Gthα) and of the specific follicle-stimulating hormone β-subunit (ag-Fshβ) of the giant Amazonian fish Arapaima gigas have been previously isolated by our research group. A synthesis of ag-Fsh in HEK293 cells and its purification and preliminary characterization were also carried out. In the present work, ag-Fsh was obtained and purified from the same host cells, and an extensive physical chemical characterization was performed via RP-HPLC, HPSEC, and MALDI-TOF-MS. Ag-Fsh, when compared to human FSH (hFSH), showed a higher hydrophobicity by RP-HPLC and a higher molecular mass (MM) via HPSEC. The same higher MM was also confirmed via MALDI-TOF-MS: 35,353 Da for ag-FSH against 31,969 Da for hFSH. Its immunological activity was also confirmed via an hFSH ELISA, in comparison with the highly purified pituitary preparation of hFSH-AFP7298A, from the National Hormone and Pituitary Program (NHPP-USA), with a clear response that was, however, 1560-fold lower when compared to the purest preparation. Finally, an in vitro bioassay, based on the stimulated release of 11-ketotestosterone (11-KT) from immature A. gigas testis, quantified ag-Fsh biological activity in comparison with human chorionic gonadotropin (hCG) and with human pituitary FSH-AFP7298A, showing a potency clearly higher than that of hCG. This suggests that injections of ag-Fsh in A. gigas and ag-Fsh cDNA gene therapy applications could be used for improving the reproductive functions of this threatened species. | |
| dc.language.iso | eng | |
| dc.rights | openAccess | |
| dc.subject | Cetotestosterona | |
| dc.title | Extensive characterization of Arapaima gigas follicle-stimulating hormone (ag-Fsh) synthesized in HEK293 cells. | |
| dc.type | Artigo de periódico | |
| dc.subject.thesagro | Hormônio | |
| dc.subject.nalthesaurus | Arapaima gigas | |
| riaa.ainfo.id | 1181967 | |
| riaa.ainfo.lastupdate | 2025-11-27 | |
| dc.identifier.doi | https://doi.org/10.3390/fishes10120607 | |
| dc.contributor.institution | ELIANA R. LIMA, UNIVERSIDADE DE SÃO PAULO; THAIS C. A. SEVILHANO, UNIVERSIDADE DE SÃO PAULO; THAIS C. FEITOSA, UNIVERSIDADE DE SÃO PAULO; JOÃO E. OLIVEIRA, UNIVERSIDADE DE SÃO PAULO; MIRIAM F. SUZUKI, UNIVERSIDADE DE SÃO PAULO; LUCAS SIMON TORATI, CNPASA; PAOLO BARTOLINI, UNIVERSIDADE DE SÃO PAULO; CIBELE N. PERONI, UNIVERSIDADE DE SÃO PAULO. | |
| Aparece en las colecciones: | Artigo em periódico indexado (CNPASA)  | |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| ExtensiveCharacterizationArapaima.pdf | 1,13 MB | Adobe PDF |  Visualizar/Abrir |
