Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1181967
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dc.contributor.authorLIMA, E. R.
dc.contributor.authorSEVILHANO, T. C. A.
dc.contributor.authorFEITOSA, T. C.
dc.contributor.authorOLIVEIRA, J. E.
dc.contributor.authorSUZUKI, M. F.
dc.contributor.authorTORATI, L. S.
dc.contributor.authorBARTOLINI, P.
dc.contributor.authorPERONI, C. N.
dc.date.accessioned2025-11-27T13:48:56Z-
dc.date.available2025-11-27T13:48:56Z-
dc.date.created2025-11-27
dc.date.issued2025
dc.identifier.citationFishes, v. 10, n. 12, p. 607, 2025.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1181967-
dc.descriptionThe cDNA sequences of the common gonadotrophic hormone α-subunit (ag-Gthα) and of the specific follicle-stimulating hormone β-subunit (ag-Fshβ) of the giant Amazonian fish Arapaima gigas have been previously isolated by our research group. A synthesis of ag-Fsh in HEK293 cells and its purification and preliminary characterization were also carried out. In the present work, ag-Fsh was obtained and purified from the same host cells, and an extensive physical chemical characterization was performed via RP-HPLC, HPSEC, and MALDI-TOF-MS. Ag-Fsh, when compared to human FSH (hFSH), showed a higher hydrophobicity by RP-HPLC and a higher molecular mass (MM) via HPSEC. The same higher MM was also confirmed via MALDI-TOF-MS: 35,353 Da for ag-FSH against 31,969 Da for hFSH. Its immunological activity was also confirmed via an hFSH ELISA, in comparison with the highly purified pituitary preparation of hFSH-AFP7298A, from the National Hormone and Pituitary Program (NHPP-USA), with a clear response that was, however, 1560-fold lower when compared to the purest preparation. Finally, an in vitro bioassay, based on the stimulated release of 11-ketotestosterone (11-KT) from immature A. gigas testis, quantified ag-Fsh biological activity in comparison with human chorionic gonadotropin (hCG) and with human pituitary FSH-AFP7298A, showing a potency clearly higher than that of hCG. This suggests that injections of ag-Fsh in A. gigas and ag-Fsh cDNA gene therapy applications could be used for improving the reproductive functions of this threatened species.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectCetotestosterona
dc.titleExtensive characterization of Arapaima gigas follicle-stimulating hormone (ag-Fsh) synthesized in HEK293 cells.
dc.typeArtigo de periódico
dc.subject.thesagroHormônio
dc.subject.nalthesaurusArapaima gigas
riaa.ainfo.id1181967
riaa.ainfo.lastupdate2025-11-27
dc.identifier.doihttps://doi.org/10.3390/fishes10120607
dc.contributor.institutionELIANA R. LIMA, UNIVERSIDADE DE SÃO PAULO; THAIS C. A. SEVILHANO, UNIVERSIDADE DE SÃO PAULO; THAIS C. FEITOSA, UNIVERSIDADE DE SÃO PAULO; JOÃO E. OLIVEIRA, UNIVERSIDADE DE SÃO PAULO; MIRIAM F. SUZUKI, UNIVERSIDADE DE SÃO PAULO; LUCAS SIMON TORATI, CNPASA; PAOLO BARTOLINI, UNIVERSIDADE DE SÃO PAULO; CIBELE N. PERONI, UNIVERSIDADE DE SÃO PAULO.
Appears in Collections:Artigo em periódico indexado (CNPASA)

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