Title:	Influence of autochthonous pathogens on fish adaptation to new rearing conditions after experiencing post-transport stress: a neglected factor in quarantine protocol development.
Authors:	PAIXÃO, P. E. G. NOGUEIRA FILHO, R. M. ABE, H. A. MADI, R. R. SANTOS, C. C. M. SOUSA, N. da C. COUTO, M. V. S. do SANTOS, H. L. SANTANA, F. S. CARVALHO, A. S. ARAUJO, J. M. FUJIMOTO, R. Y.
Affiliation:	PETERSON EMMANUEL GUIMARÃES PAIXÃO; RICARDO MARQUES NOGUEIRA FILHO; HIGO ANDRADE ABE, UNIVERSIDADE FEDERAL DA BAHIA; RUBENS RISCALA MADI, UNIVERSIDADE TIRADENTES; CINDY CAROLINE MOURA SANTOS, UNIVERSIDADE TIRADENTES; NATALINO DA COSTA SOUSA, INTITUTO FEDERAL DO MATO GROSSO DO SUL; MÁRCIA VALÉRIA SILVA DO COUTO, UNIVERSIDADE FEDERAL RURAL DO AMAZONAS; HUGO LEANDRO SANTOS, UNIVERSIDADE FEDERAL DE SERGIPE; FABRICIO SA SANTANA, UNIVERSIDADE FEDERAL DE SERGIPE; AMANDA SILVA CARVALHO, UNIVERSIDADE FEDERAL DE SERGIPE; JULIANA MATOS ARAUJO; RODRIGO YUDI FUJIMOTO, CPATC.
Date Issued:	2025
Citation:	Aquaculture, v. 604, 742378, Jun., 2025.
Description:	Best management practices such as acclimation and quarantine are essential to ensure adaptation of fish after transport, reduce stress and prevent the spread of the pathogens. However, important pathogens in fish farms are opportunistic and they are naturally presents in fish farms, and there is no information about their influence in fish adaptation to a new rearing condition, especially for tropical fish Colossoma macropomum. Therefore, this study evaluated the presence of pathogens in quarantine procedure for fish tambaqui C. macropomum. The fish were submitted to stress conditions in a “transport stress simulator” andthen quarantined into sterile and contaminated tanks. In contaminated tanks were inoculated Aeromonas hydrophila and Saprolegnia parasitica. Concurrently, two different stocking densities (1 and 2g/L of fish) were also evaluated for 45 days. Blood analysis was performed before transport stress (baseline values) and at 01, 15 and 45 days of quarantine, determining the biochemical parameters, erythrogram, leukogram and hematimetric indices. As the main result, glucose and cortisol increased in the first day of quarantine, but reduced over time, showing adaptation to new environmental rearing in sterile tanks regardless stocking density. However, there was an additional effect due to presence of pathogens where fish into the contaminated tanks demonstrated high values of cortisol, glucose, hemoglobin, lactate, hematocrit, MCH, neutrophils, basophils and LG-PAS cells, and reduction of lymphocytes and thrombocytes, which reflected in clinical signs of bacterial infection (erosion of fins, necrosis and ascites) after 15 days. At the end of experiment (45 days), fish into contaminated tanks at high stocking density presented accumulated mortality of 9% caused by Aeromonas hydrophila. As conclusion, the pathogen influences the tambaqui adaptation regardless the stocking density reflecting in quarantine with at least 30 days to observe clinical signs.
Thesagro:	Peixe Transporte Patógeno Tambaqui
NAL Thesaurus:	Fish Pathogens
DOI:	https://doi.org/10.1016/j.aquaculture.2025.742378
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPATC)